Cloning, expression, purification, and preliminary characterization of single-crystal X-ray diffraction of glucosyltransferase B of streptococcus mutans

Joshua L. Mieher; Norbert Schormann; Manisha Patel; Hui Wu; Champion Deivanayagam

doi:10.1177/1934578X19849339

Cloning, expression, purification, and preliminary characterization of single-crystal X-ray diffraction of glucosyltransferase B of streptococcus mutans

Joshua L. Mieher
, Norbert Schormann
, Manisha Patel
, Hui Wu
, Champion Deivanayagam

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Dental caries characterized by acid damage of tooth enamel is a persistent disease that begins with the formation of biofilms on the tooth surface. The secreted glucosyltransferases enable Streptococcus mutans to synthesize extracellular glucan polymers using ingested starch within the oral cavity, which eventually results in the production of acid, a contributing factor to cariogenesis. In this paper, we report the cloning, expression, purification, crystallization, and preliminary X-ray diffraction characterization of glucosyltransferase B.

Original language	English (US)
Journal	Natural Product Communications
Volume	14
Issue number	5
DOIs	https://doi.org/10.1177/1934578X19849339
State	Published - May 2019
Externally published	Yes

Funding

The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: HW and CD were partially supported by NIH/NIDCR R01DE017954. JLM is a T-90 Fellow and is supported by NIH/NIDCR T-90DE022736-06.

Funders	Funder number
NIH-NIDCR	T-90DE022736-06, R01DE017954

Keywords

Biofilm
Dental caries
Glucans
Glucosyltransferase

ASJC Scopus subject areas

Drug Discovery
Complementary and alternative medicine
Plant Science
Pharmacology

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10.1177/1934578X19849339

Cite this

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title = "Cloning, expression, purification, and preliminary characterization of single-crystal X-ray diffraction of glucosyltransferase B of streptococcus mutans",

abstract = "Dental caries characterized by acid damage of tooth enamel is a persistent disease that begins with the formation of biofilms on the tooth surface. The secreted glucosyltransferases enable Streptococcus mutans to synthesize extracellular glucan polymers using ingested starch within the oral cavity, which eventually results in the production of acid, a contributing factor to cariogenesis. In this paper, we report the cloning, expression, purification, crystallization, and preliminary X-ray diffraction characterization of glucosyltransferase B.",

keywords = "Biofilm, Dental caries, Glucans, Glucosyltransferase",

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volume = "14",

journal = "Natural Product Communications",

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AU - Mieher, Joshua L.

AU - Schormann, Norbert

AU - Patel, Manisha

AU - Wu, Hui

AU - Deivanayagam, Champion

N1 - Publisher Copyright: © The Author(s) 2019

PY - 2019/5

Y1 - 2019/5

N2 - Dental caries characterized by acid damage of tooth enamel is a persistent disease that begins with the formation of biofilms on the tooth surface. The secreted glucosyltransferases enable Streptococcus mutans to synthesize extracellular glucan polymers using ingested starch within the oral cavity, which eventually results in the production of acid, a contributing factor to cariogenesis. In this paper, we report the cloning, expression, purification, crystallization, and preliminary X-ray diffraction characterization of glucosyltransferase B.

AB - Dental caries characterized by acid damage of tooth enamel is a persistent disease that begins with the formation of biofilms on the tooth surface. The secreted glucosyltransferases enable Streptococcus mutans to synthesize extracellular glucan polymers using ingested starch within the oral cavity, which eventually results in the production of acid, a contributing factor to cariogenesis. In this paper, we report the cloning, expression, purification, crystallization, and preliminary X-ray diffraction characterization of glucosyltransferase B.

KW - Biofilm

KW - Dental caries

KW - Glucans

KW - Glucosyltransferase

UR - https://www.scopus.com/pages/publications/85066406613

UR - https://www.scopus.com/pages/publications/85066406613#tab=citedBy

U2 - 10.1177/1934578X19849339

DO - 10.1177/1934578X19849339

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VL - 14

JO - Natural Product Communications

JF - Natural Product Communications

IS - 5

ER -

Cloning, expression, purification, and preliminary characterization of single-crystal X-ray diffraction of glucosyltransferase B of streptococcus mutans

Abstract

Funding

Keywords

ASJC Scopus subject areas

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