Coactivation of multiple tightly coupled calcium channels triggers spontaneous release of GABA

Courtney Williams; Wenyan Chen; Chia Hsueh Lee; Daniel Yaeger; Nicholas P. Vyleta; Stephen M. Smith

doi:10.1038/nn.3162

Coactivation of multiple tightly coupled calcium channels triggers spontaneous release of GABA

Courtney Williams, Wenyan Chen, Chia Hsueh Lee, Daniel Yaeger, Nicholas P. Vyleta, Stephen M. Smith

Pulmonary and Critical Care Medicine

Research output: Contribution to journal › Article › peer-review

44 Scopus citations

Abstract

Voltage-activated Ca ²⁺ channels (VACCs) mediate Ca ²⁺ influx to trigger action potentialg-evoked neurotransmitter release, but the mechanism by which Ca ²⁺ regulates spontaneous transmission is unclear. We found that VACCs are the major physiological triggers for spontaneous release at mouse neocortical inhibitory synapses. Moreover, despite the absence of a synchronizing action potential, we found that spontaneous fusion of a GABA-containing vesicle required the activation of multiple tightly coupled VACCs of variable type.

Original language	English (US)
Pages (from-to)	1195-1197
Number of pages	3
Journal	Nature Neuroscience
Volume	15
Issue number	9
DOIs	https://doi.org/10.1038/nn.3162
State	Published - Sep 2012

ASJC Scopus subject areas

General Neuroscience

Access to Document

10.1038/nn.3162

Cite this

@article{9b39ec5cc2524623b9da24b0c6fdf6de,

title = "Coactivation of multiple tightly coupled calcium channels triggers spontaneous release of GABA",

abstract = "Voltage-activated Ca 2+ channels (VACCs) mediate Ca 2+ influx to trigger action potentialg-evoked neurotransmitter release, but the mechanism by which Ca 2+ regulates spontaneous transmission is unclear. We found that VACCs are the major physiological triggers for spontaneous release at mouse neocortical inhibitory synapses. Moreover, despite the absence of a synchronizing action potential, we found that spontaneous fusion of a GABA-containing vesicle required the activation of multiple tightly coupled VACCs of variable type.",

author = "Courtney Williams and Wenyan Chen and Lee, {Chia Hsueh} and Daniel Yaeger and Vyleta, {Nicholas P.} and Smith, {Stephen M.}",

note = "Funding Information: We thank M. Andresen and K. Khodakhah for helpful comments. The work was supported by the US National Institutes of Health (DA027110 and GM097433) and OCTRI. C.W. and N.P.V. were supported by a grant from the National Heart, Lung, and Blood Institute (T32HL033808).",

year = "2012",

month = sep,

doi = "10.1038/nn.3162",

language = "English (US)",

volume = "15",

pages = "1195--1197",

journal = "Nature Neuroscience",

issn = "1097-6256",

publisher = "Nature Publishing Group",

number = "9",

}

TY - JOUR

T1 - Coactivation of multiple tightly coupled calcium channels triggers spontaneous release of GABA

AU - Williams, Courtney

AU - Chen, Wenyan

AU - Lee, Chia Hsueh

AU - Yaeger, Daniel

AU - Vyleta, Nicholas P.

AU - Smith, Stephen M.

N1 - Funding Information: We thank M. Andresen and K. Khodakhah for helpful comments. The work was supported by the US National Institutes of Health (DA027110 and GM097433) and OCTRI. C.W. and N.P.V. were supported by a grant from the National Heart, Lung, and Blood Institute (T32HL033808).

PY - 2012/9

Y1 - 2012/9

N2 - Voltage-activated Ca 2+ channels (VACCs) mediate Ca 2+ influx to trigger action potentialg-evoked neurotransmitter release, but the mechanism by which Ca 2+ regulates spontaneous transmission is unclear. We found that VACCs are the major physiological triggers for spontaneous release at mouse neocortical inhibitory synapses. Moreover, despite the absence of a synchronizing action potential, we found that spontaneous fusion of a GABA-containing vesicle required the activation of multiple tightly coupled VACCs of variable type.

AB - Voltage-activated Ca 2+ channels (VACCs) mediate Ca 2+ influx to trigger action potentialg-evoked neurotransmitter release, but the mechanism by which Ca 2+ regulates spontaneous transmission is unclear. We found that VACCs are the major physiological triggers for spontaneous release at mouse neocortical inhibitory synapses. Moreover, despite the absence of a synchronizing action potential, we found that spontaneous fusion of a GABA-containing vesicle required the activation of multiple tightly coupled VACCs of variable type.

UR - http://www.scopus.com/inward/record.url?scp=84866134869&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84866134869&partnerID=8YFLogxK

U2 - 10.1038/nn.3162

DO - 10.1038/nn.3162

M3 - Article

C2 - 22842148

AN - SCOPUS:84866134869

SN - 1097-6256

VL - 15

SP - 1195

EP - 1197

JO - Nature Neuroscience

JF - Nature Neuroscience

IS - 9

ER -

Coactivation of multiple tightly coupled calcium channels triggers spontaneous release of GABA

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this