Coactivation of multiple tightly coupled calcium channels triggers spontaneous release of GABA

Courtney Williams; Wenyan Chen; Chia Hsueh Lee; Daniel Yaeger; Nicholas P. Vyleta; Stephen M. Smith

doi:10.1038/nn.3162

Coactivation of multiple tightly coupled calcium channels triggers spontaneous release of GABA

Courtney Williams, Wenyan Chen, Chia Hsueh Lee, Daniel Yaeger, Nicholas P. Vyleta, Stephen M. Smith

Pulmonary and Critical Care Medicine

Research output: Contribution to journal › Article › peer-review

41 Scopus citations

Abstract

Voltage-activated Ca ²⁺ channels (VACCs) mediate Ca ²⁺ influx to trigger action potentialg-evoked neurotransmitter release, but the mechanism by which Ca ²⁺ regulates spontaneous transmission is unclear. We found that VACCs are the major physiological triggers for spontaneous release at mouse neocortical inhibitory synapses. Moreover, despite the absence of a synchronizing action potential, we found that spontaneous fusion of a GABA-containing vesicle required the activation of multiple tightly coupled VACCs of variable type.

Original language	English (US)
Pages (from-to)	1195-1197
Number of pages	3
Journal	Nature Neuroscience
Volume	15
Issue number	9
DOIs	https://doi.org/10.1038/nn.3162
State	Published - Sep 2012

ASJC Scopus subject areas

Neuroscience(all)

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title = "Coactivation of multiple tightly coupled calcium channels triggers spontaneous release of GABA",

abstract = "Voltage-activated Ca 2+ channels (VACCs) mediate Ca 2+ influx to trigger action potentialg-evoked neurotransmitter release, but the mechanism by which Ca 2+ regulates spontaneous transmission is unclear. We found that VACCs are the major physiological triggers for spontaneous release at mouse neocortical inhibitory synapses. Moreover, despite the absence of a synchronizing action potential, we found that spontaneous fusion of a GABA-containing vesicle required the activation of multiple tightly coupled VACCs of variable type.",

author = "Courtney Williams and Wenyan Chen and Lee, {Chia Hsueh} and Daniel Yaeger and Vyleta, {Nicholas P.} and Smith, {Stephen M.}",

note = "Funding Information: We thank M. Andresen and K. Khodakhah for helpful comments. The work was supported by the US National Institutes of Health (DA027110 and GM097433) and OCTRI. C.W. and N.P.V. were supported by a grant from the National Heart, Lung, and Blood Institute (T32HL033808).",

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AU - Williams, Courtney

AU - Chen, Wenyan

AU - Lee, Chia Hsueh

AU - Yaeger, Daniel

AU - Vyleta, Nicholas P.

AU - Smith, Stephen M.

N1 - Funding Information: We thank M. Andresen and K. Khodakhah for helpful comments. The work was supported by the US National Institutes of Health (DA027110 and GM097433) and OCTRI. C.W. and N.P.V. were supported by a grant from the National Heart, Lung, and Blood Institute (T32HL033808).

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Coactivation of multiple tightly coupled calcium channels triggers spontaneous release of GABA

Abstract

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