Commensal orthologs of the human autoantigen Ro60 as triggers of autoimmunity in lupus

Teri M. Greiling, Carina Dehner, Xinguo Chen, Kevin Hughes, Alonso J. Iñiguez, Marco Boccitto, Daniel Zegarra Ruiz, Stephen C. Renfroe, Silvio M. Vieira, William E. Ruff, Soyeong Sim, Christina Kriegel, Julia Glanternik, Xindi Chen, Michael Girardi, Patrick Degnan, Karen H. Costenbader, Andrew L. Goodman, Sandra L. Wolin, Martin A. Kriegel

Research output: Contribution to journalArticlepeer-review

173 Scopus citations


The earliest autoantibodies in lupus are directed against the RNA binding autoantigen Ro60, but the triggers against this evolutionarily conserved antigen remain elusive. We identified Ro60 orthologs in a subset of human skin, oral, and gut commensal bacterial species and confirmed the presence of these orthologs in patients with lupus and healthy controls. Thus, we hypothesized that commensal Ro60 orthologs may trigger autoimmunity via cross-reactivity in genetically susceptible individuals. Sera from human anti-Ro60-positive lupus patients immunoprecipitated commensal Ro60 ribonucleoproteins. Human Ro60 autoantigen-specific CD4 memory T cell clones from lupus patients were activated by skin and mucosal Ro60-containing bacteria, supporting T cell cross-reactivity in humans. Further, germ-free mice spontaneously initiated anti-human Ro60 T and B cell responses and developed glomerular immune complex deposits after monocolonization with a Ro60 ortholog-containing gut commensal, linking anti-Ro60 commensal responses in vivo with the production of human Ro60 autoantibodies and signs of autoimmunity. Together, these data support that colonization with autoantigen ortholog-producing commensal species may initiate and sustain chronic autoimmunity in genetically predisposed individuals. The concept of commensal ortholog cross-reactivity may apply more broadly to autoimmune diseases and lead to novel treatment approaches aimed at defined commensal species.

Original languageEnglish (US)
Article numbereaan2306
JournalScience translational medicine
Issue number434
StatePublished - Mar 28 2018
Externally publishedYes

ASJC Scopus subject areas

  • Medicine(all)


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