TY - JOUR
T1 - Comparative Genomic Characterization of the Multimammate Mouse Mastomys coucha
AU - Hardin, Aaron
AU - Nevonen, Kimberly A.
AU - Eckalbar, Walter L.
AU - Carbone, Lucia
AU - Ahituv, Nadav
N1 - Publisher Copyright:
© 2019 The Author(s) 2019. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution.
PY - 2019/12/1
Y1 - 2019/12/1
N2 - Mastomys are the most widespread African rodent and carriers of various diseases such as the plague or Lassa virus. In addition, mastomys have rapidly gained a large number of mammary glands. Here, we generated a genome, variome, and transcriptomes for Mastomys coucha. As mastomys diverged at similar times from mouse and rat, we demonstrate their utility as a comparative genomic tool for these commonly used animal models. Furthermore, we identified over 500 mastomys accelerated regions, often residing near important mammary developmental genes or within their exons leading to protein sequence changes. Functional characterization of a noncoding mastomys accelerated region, located in the HoxD locus, showed enhancer activity in mouse developing mammary glands. Combined, our results provide genomic resources for mastomys and highlight their potential both as a comparative genomic tool and for the identification of mammary gland number determining factors.
AB - Mastomys are the most widespread African rodent and carriers of various diseases such as the plague or Lassa virus. In addition, mastomys have rapidly gained a large number of mammary glands. Here, we generated a genome, variome, and transcriptomes for Mastomys coucha. As mastomys diverged at similar times from mouse and rat, we demonstrate their utility as a comparative genomic tool for these commonly used animal models. Furthermore, we identified over 500 mastomys accelerated regions, often residing near important mammary developmental genes or within their exons leading to protein sequence changes. Functional characterization of a noncoding mastomys accelerated region, located in the HoxD locus, showed enhancer activity in mouse developing mammary glands. Combined, our results provide genomic resources for mastomys and highlight their potential both as a comparative genomic tool and for the identification of mammary gland number determining factors.
KW - Comparative genomics
KW - Mastomys
KW - mammary glands
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U2 - 10.1093/molbev/msz188
DO - 10.1093/molbev/msz188
M3 - Article
C2 - 31424545
AN - SCOPUS:85075813973
SN - 0737-4038
VL - 36
SP - 2805
EP - 2812
JO - Molecular Biology and Evolution
JF - Molecular Biology and Evolution
IS - 12
ER -