Comparison of murine Supt4h and a nearly identical expressed, processed gene: Evidence of sequence conservation through gene conversion extending into the untranslated regions

Pei Wen Chiang; Ruobo Zhang; Lisa Stubbs; Liang Zhang; Li Zhu; David M. Kurnit

doi:10.1093/nar/26.21.4960

Comparison of murine Supt4h and a nearly identical expressed, processed gene: Evidence of sequence conservation through gene conversion extending into the untranslated regions

Pei Wen Chiang
, Ruobo Zhang
, Lisa Stubbs
, Liang Zhang
, Li Zhu
, David M. Kurnit

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

We show herein the transcription of a processed gene that originated from a spliced transcript. Recently, we isolated the human and murine homologues of the yeast chromatin protein, SPT4. The Supt4h gene is spliced normally from five exons encoded by chromosome 11. Here we show that a related sequence on chromosome 10 encodes Supt4h2, a processed intronless gene (with a polyA tail and a tandemly-duplicated 13 bp insertion site in the genome) with a different 5' control region. Both the spliced gene, Supt4h, and the processed gene, Supt4h2, are expressed in each of four tissues we examined. Supt4h2 encodes a 117 amino acid protein nearly identical to the Supt4h gene product with only one amino acid difference, indicating extreme conservation of this expressed processed gene with the spliced gene over evolutionary time. This illustrates another potential complexity of the mammalian genome, i.e. the use of a processed gene under the control of a different promoter region than the spliced gene.

Original language	English (US)
Pages (from-to)	4960-4964
Number of pages	5
Journal	Nucleic acids research
Volume	26
Issue number	21
DOIs	https://doi.org/10.1093/nar/26.21.4960
State	Published - Nov 1 1998
Externally published	Yes

Funding

L.S. was supported by a grant from the US Department of Energy (awarded under contract DE-AC05-96OR22464 with Lockheed-Martin Energy Systems, Inc.). P.-W.C. and D.M.K. were supported by NIH grants R01 HL50025 and R42 CA77235. We thank Beverly Selmer for expert technical assistance, and Dr Micheal Mucenski and Hsi-Hsien Lin for kindly providing the Myb gene probe. We also thank L. Rowe and M. Barter for Figure 2 and the University of Michigan DNA sequencing core.

Funders	Funder number
Lockheed-Martin Energy Systems, Inc.
Author National Institutes of Health National Institutes of Health National Institutes of Health National Institutes of Health The Bev Hartig Huntington's Disease Foundation National Institutes of Health	R01 HL50025
U.S. Department of Energy	DE-AC05-96OR22464
National Institute of Health-National Cancer Institute	R42CA077235

ASJC Scopus subject areas

Genetics

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Cite this

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title = "Comparison of murine Supt4h and a nearly identical expressed, processed gene: Evidence of sequence conservation through gene conversion extending into the untranslated regions",

abstract = "We show herein the transcription of a processed gene that originated from a spliced transcript. Recently, we isolated the human and murine homologues of the yeast chromatin protein, SPT4. The Supt4h gene is spliced normally from five exons encoded by chromosome 11. Here we show that a related sequence on chromosome 10 encodes Supt4h2, a processed intronless gene (with a polyA tail and a tandemly-duplicated 13 bp insertion site in the genome) with a different 5' control region. Both the spliced gene, Supt4h, and the processed gene, Supt4h2, are expressed in each of four tissues we examined. Supt4h2 encodes a 117 amino acid protein nearly identical to the Supt4h gene product with only one amino acid difference, indicating extreme conservation of this expressed processed gene with the spliced gene over evolutionary time. This illustrates another potential complexity of the mammalian genome, i.e. the use of a processed gene under the control of a different promoter region than the spliced gene.",

author = "Chiang, \{Pei Wen\} and Ruobo Zhang and Lisa Stubbs and Liang Zhang and Li Zhu and Kurnit, \{David M.\}",

note = "Funding Information: L.S. was supported by a grant from the US Department of Energy (awarded under contract DE-AC05-96OR22464 with Lockheed-Martin Energy Systems, Inc.). P.-W.C. and D.M.K. were supported by NIH grants R01 HL50025 and R42 CA77235. We thank Beverly Selmer for expert technical assistance, and Dr Micheal Mucenski and Hsi-Hsien Lin for kindly providing the Myb gene probe. We also thank L. Rowe and M. Barter for Figure 2 and the University of Michigan DNA sequencing core.",

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N2 - We show herein the transcription of a processed gene that originated from a spliced transcript. Recently, we isolated the human and murine homologues of the yeast chromatin protein, SPT4. The Supt4h gene is spliced normally from five exons encoded by chromosome 11. Here we show that a related sequence on chromosome 10 encodes Supt4h2, a processed intronless gene (with a polyA tail and a tandemly-duplicated 13 bp insertion site in the genome) with a different 5' control region. Both the spliced gene, Supt4h, and the processed gene, Supt4h2, are expressed in each of four tissues we examined. Supt4h2 encodes a 117 amino acid protein nearly identical to the Supt4h gene product with only one amino acid difference, indicating extreme conservation of this expressed processed gene with the spliced gene over evolutionary time. This illustrates another potential complexity of the mammalian genome, i.e. the use of a processed gene under the control of a different promoter region than the spliced gene.

AB - We show herein the transcription of a processed gene that originated from a spliced transcript. Recently, we isolated the human and murine homologues of the yeast chromatin protein, SPT4. The Supt4h gene is spliced normally from five exons encoded by chromosome 11. Here we show that a related sequence on chromosome 10 encodes Supt4h2, a processed intronless gene (with a polyA tail and a tandemly-duplicated 13 bp insertion site in the genome) with a different 5' control region. Both the spliced gene, Supt4h, and the processed gene, Supt4h2, are expressed in each of four tissues we examined. Supt4h2 encodes a 117 amino acid protein nearly identical to the Supt4h gene product with only one amino acid difference, indicating extreme conservation of this expressed processed gene with the spliced gene over evolutionary time. This illustrates another potential complexity of the mammalian genome, i.e. the use of a processed gene under the control of a different promoter region than the spliced gene.

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Comparison of murine Supt4h and a nearly identical expressed, processed gene: Evidence of sequence conservation through gene conversion extending into the untranslated regions

Abstract

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