Abstract
The RVL3 VL30 enhancer is an LTR-derived triple direct repeat of 35 base pairs that mediates gene induction in response to several different intracellular signaling pathways. Using mobility shift assays, methylation interference and DNase I footprinting, we have investigated the physical interactions between the RVL-3 enhancer and components of nuclear extracts from Rat-1 cells. Each enhancer repeat unit contains a single binding site. Our studies suggest that binding to the double or triple repeat enhancer is cooperative, involving simultaneous occupation of two sites, with a preference for adjacent sites. Binding cooperativity would have implications for the mechanism of gene activation directed from the native VL30 enhancer.
Original language | English (US) |
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Pages (from-to) | 55-65 |
Number of pages | 11 |
Journal | Biochimica et Biophysica Acta - Gene Structure and Expression |
Volume | 1307 |
Issue number | 1 |
DOIs | |
State | Published - Jun 3 1996 |
Keywords
- CREB/JUN
- Cooperative binding
- Enhancer
- VL30
ASJC Scopus subject areas
- Structural Biology
- Biophysics
- Biochemistry
- Genetics