Confocal laser scanning microscopy using scattering as the contrast mechanism

Steven L. Jacques

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Scopus citations


Confocal laser scanning microscopy (CLSM) is best known and widely used for fluorescence imaging. However, any commercial CLSM can be operated in reflectance mode by setting the microscope's detector to accept the excitation laser wavelength. In this mode, the images are based on the scattering properties of the cell or tissue. This chapter discusses reflectance-mode CLSM (rCLSM), the mechanisms of contrast involved in such images, and examples of how rCLSM is being used to study tissue properties.

Original languageEnglish (US)
Title of host publicationHandbook of Coherent-Domain Optical Methods: Biomedical Diagnostics, Environmental Monitoring, and Materials Science:: Second Edition
PublisherSpringer New York
Number of pages15
ISBN (Electronic)9781461451761
ISBN (Print)9781461451754
StatePublished - Jan 1 2013

ASJC Scopus subject areas

  • General Biochemistry, Genetics and Molecular Biology


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