Construction of combinatorial immune library of single chain human antibodies to orthopoxviruses and selection from this library antibodies to recombinant protein prA30L of variola virus

V. V. Dubrovskaia; A. B. Ulitin; A. G. Laman; I. P. Gileva; N. I. Bormotov; A. A. Il'ichev; F. A. Brovko; S. N. Shchelkunov; E. F. Belanov; N. V. Tikunova

Construction of combinatorial immune library of single chain human antibodies to orthopoxviruses and selection from this library antibodies to recombinant protein prA30L of variola virus

V. V. Dubrovskaia, A. B. Ulitin, A. G. Laman, I. P. Gileva, N. I. Bormotov, A. A. Il'ichev, F. A. Brovko, S. N. Shchelkunov, E. F. Belanov, N. V. Tikunova

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

A combinatorial immune library of human single-chain antibody fragments (scFv) was constructed on the base of genes encoding variable domains of heavy and light chains of immunoglobulins cloned from the lymphocytes of four vaccinia virus (VACV) vaccinated donors. The size of the library was 3 x 10(7) independent clones. After the library was enriched with the clones producing scFv against recombinant analogue of variola virus surface protein prA30L, a panel of unique antibodies specific to both prA30L and VACV was selected from the library. A plaque reduction neutralization test was performed for all selected antibodies and two antibodies were shown to be able to neutralize plaque formation of VACV in Vero E6 cells monolayer. Binding specificities of these antibodies were confirmed using ELISA and Western blot analysis. To determine the amino acid sequences of neutralizing antibodies their genes were sequenced.

Original language	English (US)
Pages (from-to)	173-185
Number of pages	13
Journal	Molekuliarnaia biologiia
Volume	41
Issue number	1
State	Published - Jan 2007
Externally published	Yes

ASJC Scopus subject areas

General Medicine

Cite this

Dubrovskaia, V. V., Ulitin, A. B., Laman, A. G., Gileva, I. P., Bormotov, N. I., Il'ichev, A. A., Brovko, F. A., Shchelkunov, S. N., Belanov, E. F., & Tikunova, N. V. (2007). Construction of combinatorial immune library of single chain human antibodies to orthopoxviruses and selection from this library antibodies to recombinant protein prA30L of variola virus. Molekuliarnaia biologiia, 41(1), 173-185.

Construction of combinatorial immune library of single chain human antibodies to orthopoxviruses and selection from this library antibodies to recombinant protein prA30L of variola virus. / Dubrovskaia, V. V.; Ulitin, A. B.; Laman, A. G. et al.
In: Molekuliarnaia biologiia, Vol. 41, No. 1, 01.2007, p. 173-185.

Research output: Contribution to journal › Article › peer-review

Dubrovskaia, VV, Ulitin, AB, Laman, AG, Gileva, IP, Bormotov, NI, Il'ichev, AA, Brovko, FA, Shchelkunov, SN, Belanov, EF & Tikunova, NV 2007, 'Construction of combinatorial immune library of single chain human antibodies to orthopoxviruses and selection from this library antibodies to recombinant protein prA30L of variola virus', Molekuliarnaia biologiia, vol. 41, no. 1, pp. 173-185.

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AU - Gileva, I. P.

AU - Bormotov, N. I.

AU - Il'ichev, A. A.

AU - Brovko, F. A.

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Construction of combinatorial immune library of single chain human antibodies to orthopoxviruses and selection from this library antibodies to recombinant protein prA30L of variola virus

Abstract

ASJC Scopus subject areas

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