Abstract
We have previously reported that adenovirus-mediated expression of preprocholecystokin (CCK) stimulates human and mouse islet cell proliferation. In follow-up studies, we became concerned that the CCK adenovirus might have been contaminated with a wild-type E1A-containing adenovirus. Here we show conclusively that the proliferative effects reported in the original paper in mouse and human islets were not due to CCK expression but rather to a contaminating E1Aexpressing wild-type adenovirus. We also show, however, that CCK expression does have a proliferative effect in rat islets.We hope that our report of the steps taken to detect the wild-type virus contamination, and purification of the contributing viral stocks, will be helpful to other investigators, and that our experience will serve as a cautionary tale for use of adenovirus vectors, especially for studies on cellular replication.
Original language | English (US) |
---|---|
Pages (from-to) | 464-467 |
Number of pages | 4 |
Journal | Molecular Endocrinology |
Volume | 24 |
Issue number | 2 |
DOIs | |
State | Published - Feb 2010 |
Externally published | Yes |
ASJC Scopus subject areas
- Molecular Biology
- Endocrinology