Abstract
Biosynthesis and processing of ACTH/β-lipotropin was studied in Nelson's syndrome pituitary tumor tissue grown in tnonolayer culture. Radiolabeled peptides were immunoprecipitated and fractionated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS/PAGE). Important findings include: 1) a virtual absence of 13K ACTH or 3.5K β-endorphin production; 2) evidence indicating the presence of a 24-26K ACTH and β-LPH containing intermediate (which implies a different order of processing from that reported in the mouse); 3) An extremely rapid rate of turnover and release of ACTH and β-lipotropin (β-LPH) similar to that of the mouse AtT20/D16v pituitary tumors. The latter finding is consistent with an intrinsic pituitary cell defect in the pathogenesis of this disorder.
Original language | English (US) |
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Pages (from-to) | 887-889 |
Number of pages | 3 |
Journal | Journal of Clinical Endocrinology and Metabolism |
Volume | 53 |
Issue number | 4 |
DOIs | |
State | Published - Oct 1981 |
Externally published | Yes |
ASJC Scopus subject areas
- Endocrinology, Diabetes and Metabolism
- Biochemistry
- Endocrinology
- Clinical Biochemistry
- Biochemistry, medical