Cultured human retinal pigment epithelial cells express basic fibroblast growth factor and its receptor

Mark D. Stcrnfeld; Joseph E. Robertson; Gary D. Shipley; Jeanne Tsai; James T. Rosenbaum

doi:10.3109/02713688908997395

Cultured human retinal pigment epithelial cells express basic fibroblast growth factor and its receptor

Mark D. Stcrnfeld, Joseph E. Robertson, Gary D. Shipley, Jeanne Tsai, James T. Rosenbaum

Ophthalmology

Research output: Contribution to journal › Article › peer-review

107 Scopus citations

Abstract

Basic fibroblast growth factor (bFGF) has been implicated in the maintenance of neuronal differentiation, the induction of neovascularization and intravitreal proliferative diseases. We have found that human retinal pigment epithelial (RFE) cells grown in vitro transcribe the bFGF gene and synthesize a peptide that crossreacts with anti-bFGF antibodies. In culture, these cells appear to release activity with biological and biochemical properties similar to bFGF. RPE cells have specific bFGF receptors and proliferate in response to bFGF, Thus, it is possible that the RPE cell is an important source of retinal bFGF and may respond to bFGF in an autocrine manner.

Original language	English (US)
Pages (from-to)	1029-1037
Number of pages	9
Journal	Current Eye Research
Volume	8
Issue number	10
DOIs	https://doi.org/10.3109/02713688908997395
State	Published - 1989

ASJC Scopus subject areas

Ophthalmology
Sensory Systems
Cellular and Molecular Neuroscience

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@article{f47489740bf64246a03123c63b9eea83,

title = "Cultured human retinal pigment epithelial cells express basic fibroblast growth factor and its receptor",

abstract = "Basic fibroblast growth factor (bFGF) has been implicated in the maintenance of neuronal differentiation, the induction of neovascularization and intravitreal proliferative diseases. We have found that human retinal pigment epithelial (RFE) cells grown in vitro transcribe the bFGF gene and synthesize a peptide that crossreacts with anti-bFGF antibodies. In culture, these cells appear to release activity with biological and biochemical properties similar to bFGF. RPE cells have specific bFGF receptors and proliferate in response to bFGF, Thus, it is possible that the RPE cell is an important source of retinal bFGF and may respond to bFGF in an autocrine manner.",

author = "Stcrnfeld, {Mark D.} and Robertson, {Joseph E.} and Shipley, {Gary D.} and Jeanne Tsai and Rosenbaum, {James T.}",

note = "Funding Information: ACKNOWLEDGEMENTS This work was supported by USPHS grants CA42409 (GDS), EY 07373 (JTR) and EY06484 (JTR) and performed while J.T.R. was a Dolly Green Scholar for Research to Prevent Blindness, New York, NY.The authors thank Drs. Judith Abraham and John Fiddes of California Biotechnology, Inc. for providing the bFGF cDNA, Dr. Eric Smith of the University of North Carolina for providing the bFGF antisera; and Dr. Bruce Magun of The Oregon Health Sciences University for providing the EGF.",

year = "1989",

doi = "10.3109/02713688908997395",

language = "English (US)",

volume = "8",

pages = "1029--1037",

journal = "Current Eye Research",

issn = "0271-3683",

publisher = "Informa Healthcare",

number = "10",

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TY - JOUR

T1 - Cultured human retinal pigment epithelial cells express basic fibroblast growth factor and its receptor

AU - Stcrnfeld, Mark D.

AU - Robertson, Joseph E.

AU - Shipley, Gary D.

AU - Tsai, Jeanne

AU - Rosenbaum, James T.

N1 - Funding Information: ACKNOWLEDGEMENTS This work was supported by USPHS grants CA42409 (GDS), EY 07373 (JTR) and EY06484 (JTR) and performed while J.T.R. was a Dolly Green Scholar for Research to Prevent Blindness, New York, NY.The authors thank Drs. Judith Abraham and John Fiddes of California Biotechnology, Inc. for providing the bFGF cDNA, Dr. Eric Smith of the University of North Carolina for providing the bFGF antisera; and Dr. Bruce Magun of The Oregon Health Sciences University for providing the EGF.

PY - 1989

Y1 - 1989

N2 - Basic fibroblast growth factor (bFGF) has been implicated in the maintenance of neuronal differentiation, the induction of neovascularization and intravitreal proliferative diseases. We have found that human retinal pigment epithelial (RFE) cells grown in vitro transcribe the bFGF gene and synthesize a peptide that crossreacts with anti-bFGF antibodies. In culture, these cells appear to release activity with biological and biochemical properties similar to bFGF. RPE cells have specific bFGF receptors and proliferate in response to bFGF, Thus, it is possible that the RPE cell is an important source of retinal bFGF and may respond to bFGF in an autocrine manner.

AB - Basic fibroblast growth factor (bFGF) has been implicated in the maintenance of neuronal differentiation, the induction of neovascularization and intravitreal proliferative diseases. We have found that human retinal pigment epithelial (RFE) cells grown in vitro transcribe the bFGF gene and synthesize a peptide that crossreacts with anti-bFGF antibodies. In culture, these cells appear to release activity with biological and biochemical properties similar to bFGF. RPE cells have specific bFGF receptors and proliferate in response to bFGF, Thus, it is possible that the RPE cell is an important source of retinal bFGF and may respond to bFGF in an autocrine manner.

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JF - Current Eye Research

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Cultured human retinal pigment epithelial cells express basic fibroblast growth factor and its receptor

Abstract

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