Cyclodextrin enhances spermicidal effects of magainin-2-amide

C. Wojcik; W. Sawicki; P. Marianowski; M. Benchaib; J. C. Czyba; J. F. Guerin

doi:10.1016/S0010-7824(00)00143-8

Cyclodextrin enhances spermicidal effects of magainin-2-amide

C. Wojcik
, W. Sawicki
, P. Marianowski
, M. Benchaib
, J. C. Czyba
, J. F. Guerin

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

Magainins are antimicrobial peptides with known spermicidal activity. Their activity is inhibited by cholesterol present in eukaryotic cell membranes. Pretreatment of spermatozoa with methyl-β-cyclodextrin, which extracts cholesterol from cell membranes and induces capacitation, sensitizes them to magainin-2-amide as shown by a decrease in human sperm motility determined by computer-assisted sperm analysis and a concomitant decrease in sperm viability, as measured by MitoTracker® Red CMXRos labeling. Magainin-2-amide affects mainly the fast progressive spermatozoa inducing them directly into an immotile state, without an increase in motile non-progressive and slow progressive spermatozoa. We conclude that methyl-β-cyclodextrin highly potentiates the deleterious effect of magainin-2-amide on human spermatozoa. Most probably, this effect can be explained by cholesterol extraction from sperm cell membranes. Copyright (C) 2000 Elsevier Science Inc.

Original language	English (US)
Pages (from-to)	99-103
Number of pages	5
Journal	Contraception
Volume	62
Issue number	2
DOIs	https://doi.org/10.1016/S0010-7824(00)00143-8
State	Published - 2000
Externally published	Yes

Funding

This work has been in part supported by the KBN grant 4 P05A 084 16 and ISDS 1M15/S/99 small grant of the Warsaw Medical Academy to C.W.

Funders	Funder number
KBN	4 P05A 084 16, ISDS 1M15/S/99
Warsaw Medical Academy

Keywords

Cyclodextrin
Human spermatozoa
Magainin-2-amide
Sperm motility
Sperm viability

ASJC Scopus subject areas

Reproductive Medicine
Obstetrics and Gynecology

Access to Document

10.1016/S0010-7824(00)00143-8

Cite this

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title = "Cyclodextrin enhances spermicidal effects of magainin-2-amide",

abstract = "Magainins are antimicrobial peptides with known spermicidal activity. Their activity is inhibited by cholesterol present in eukaryotic cell membranes. Pretreatment of spermatozoa with methyl-β-cyclodextrin, which extracts cholesterol from cell membranes and induces capacitation, sensitizes them to magainin-2-amide as shown by a decrease in human sperm motility determined by computer-assisted sperm analysis and a concomitant decrease in sperm viability, as measured by MitoTracker{\textregistered} Red CMXRos labeling. Magainin-2-amide affects mainly the fast progressive spermatozoa inducing them directly into an immotile state, without an increase in motile non-progressive and slow progressive spermatozoa. We conclude that methyl-β-cyclodextrin highly potentiates the deleterious effect of magainin-2-amide on human spermatozoa. Most probably, this effect can be explained by cholesterol extraction from sperm cell membranes. Copyright (C) 2000 Elsevier Science Inc.",

keywords = "Cyclodextrin, Human spermatozoa, Magainin-2-amide, Sperm motility, Sperm viability",

author = "C. Wojcik and W. Sawicki and P. Marianowski and M. Benchaib and Czyba, \{J. C.\} and Guerin, \{J. F.\}",

note = "Funding Information: This work has been in part supported by the KBN grant 4 P05A 084 16 and ISDS 1M15/S/99 small grant of the Warsaw Medical Academy to C.W.",

year = "2000",

doi = "10.1016/S0010-7824(00)00143-8",

language = "English (US)",

volume = "62",

pages = "99--103",

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T1 - Cyclodextrin enhances spermicidal effects of magainin-2-amide

AU - Wojcik, C.

AU - Sawicki, W.

AU - Marianowski, P.

AU - Benchaib, M.

AU - Czyba, J. C.

AU - Guerin, J. F.

N1 - Funding Information: This work has been in part supported by the KBN grant 4 P05A 084 16 and ISDS 1M15/S/99 small grant of the Warsaw Medical Academy to C.W.

PY - 2000

Y1 - 2000

N2 - Magainins are antimicrobial peptides with known spermicidal activity. Their activity is inhibited by cholesterol present in eukaryotic cell membranes. Pretreatment of spermatozoa with methyl-β-cyclodextrin, which extracts cholesterol from cell membranes and induces capacitation, sensitizes them to magainin-2-amide as shown by a decrease in human sperm motility determined by computer-assisted sperm analysis and a concomitant decrease in sperm viability, as measured by MitoTracker® Red CMXRos labeling. Magainin-2-amide affects mainly the fast progressive spermatozoa inducing them directly into an immotile state, without an increase in motile non-progressive and slow progressive spermatozoa. We conclude that methyl-β-cyclodextrin highly potentiates the deleterious effect of magainin-2-amide on human spermatozoa. Most probably, this effect can be explained by cholesterol extraction from sperm cell membranes. Copyright (C) 2000 Elsevier Science Inc.

AB - Magainins are antimicrobial peptides with known spermicidal activity. Their activity is inhibited by cholesterol present in eukaryotic cell membranes. Pretreatment of spermatozoa with methyl-β-cyclodextrin, which extracts cholesterol from cell membranes and induces capacitation, sensitizes them to magainin-2-amide as shown by a decrease in human sperm motility determined by computer-assisted sperm analysis and a concomitant decrease in sperm viability, as measured by MitoTracker® Red CMXRos labeling. Magainin-2-amide affects mainly the fast progressive spermatozoa inducing them directly into an immotile state, without an increase in motile non-progressive and slow progressive spermatozoa. We conclude that methyl-β-cyclodextrin highly potentiates the deleterious effect of magainin-2-amide on human spermatozoa. Most probably, this effect can be explained by cholesterol extraction from sperm cell membranes. Copyright (C) 2000 Elsevier Science Inc.

KW - Cyclodextrin

KW - Human spermatozoa

KW - Magainin-2-amide

KW - Sperm motility

KW - Sperm viability

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DO - 10.1016/S0010-7824(00)00143-8

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SN - 0010-7824

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JO - Contraception

JF - Contraception

IS - 2

ER -

Cyclodextrin enhances spermicidal effects of magainin-2-amide

Abstract

Funding

Keywords

ASJC Scopus subject areas

Access to Document

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