TY - JOUR
T1 - Cysteine protease inhibitor e64 reduces the rate of formation of selenite cataract in the whole animal
AU - Azuma, Mitsuyoshi
AU - David, Larry L.
AU - Shearer, Thomas (Tom)
N1 - Funding Information:
ACKNOWLEDGEMENTS The authors thank Jay Wright for expert technical assistance. Partially supported by NIH grants EY03600 and EY05786.
PY - 1991
Y1 - 1991
N2 - The purpose of this experiment was to test the effectiveness of E64 in prevention of selenite nuclear cataract in the whole animal. E64 is an inhibitor of cysteine proteases such as calpain (EC.3.4.22.17). In the whole animal, daily intraperitoneal injection of E64 was mildly effective in slowing the rate of formation of selenite nuclear cataract, although prevention was not permanent. Frequency of the nuclear cataract in selenite group at 5 days post selenite injection was significantly decreased from 40% to 17% in the selenite+E64 group, and the density of cataract in the Se+E64 group was reduced. However, crystallins and calpain were still degraded in the selenite+E64 group. E64 was more effective against selenite cataract when present continuously during lens culture, where it slowed the rate of formation of nuclear opacity. Amelioration of cataract occurred both in vitro and in vivo even though lens calcium concentrations were elevated. The results supported the idea that application of calpain inhibitor is beneficial hi prevention of rodent selenite cataracts.
AB - The purpose of this experiment was to test the effectiveness of E64 in prevention of selenite nuclear cataract in the whole animal. E64 is an inhibitor of cysteine proteases such as calpain (EC.3.4.22.17). In the whole animal, daily intraperitoneal injection of E64 was mildly effective in slowing the rate of formation of selenite nuclear cataract, although prevention was not permanent. Frequency of the nuclear cataract in selenite group at 5 days post selenite injection was significantly decreased from 40% to 17% in the selenite+E64 group, and the density of cataract in the Se+E64 group was reduced. However, crystallins and calpain were still degraded in the selenite+E64 group. E64 was more effective against selenite cataract when present continuously during lens culture, where it slowed the rate of formation of nuclear opacity. Amelioration of cataract occurred both in vitro and in vivo even though lens calcium concentrations were elevated. The results supported the idea that application of calpain inhibitor is beneficial hi prevention of rodent selenite cataracts.
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U2 - 10.3109/02713689109013857
DO - 10.3109/02713689109013857
M3 - Article
C2 - 1914502
AN - SCOPUS:0025779333
SN - 0271-3683
VL - 10
SP - 657
EP - 666
JO - Current Eye Research
JF - Current Eye Research
IS - 7
ER -