TY - JOUR
T1 - Defining the critical-sized defect in a rat segmental mandibulectomy model
AU - Deconde, Adam S.
AU - Lee, Matthew K.
AU - Sidell, Douglas
AU - Aghaloo, Tara
AU - Lee, Min
AU - Tetradis, Sotirios
AU - Low, Kyle
AU - Elashoff, David
AU - Grogan, Tristan
AU - Sepahdari, Ali R.
AU - John, Maie St
PY - 2014/1
Y1 - 2014/1
N2 - IMPORTANCE Advances in tissue engineering offer potential alternatives to current mandibular reconstructive techniques; however, before clinical translation of this technology, a relevant animal model must be used to validate possible interventions. OBJECTIVE To establish the critical-sized segmental mandibular defect that does not heal spontaneously in the rat mandible. DESIGN AND SETTING Prospective study of mandibular defect healing in 29 Sprague-Dawley rats in an animal laboratory. INTERVENTIONS The rats underwent creation of 1 of 4 segmental mandibular defects measuring 0, 1, 3, and 5 mm. All mandibular wounds were internally fixated with 1-mm microplates and screws and allowed to heal for 12 weeks, after which the animals were killed humanely. MAIN OUTCOMES AND MEASURES Analysis with micro-computed tomography of bony union and formation graded on semiquantitative scales. RESULTS Seven animals were included in each experimental group. No 5-mm segmental defects successfully developed bony union, whereas all 0- and 1-mm defects had continuous bony growth across the original defect on micro-computed tomography. Three of the 3-mm defects had bony continuity, and 3 had no healing of the bony wound. Bone union scores were significantly lower for the 5-mm defects compared with the 0-, 1-, and 3-mm defects (P < .01). CONCLUSIONS AND RELEVANCE The rat segmental mandible model cannot heal a 5-mm segmental mandibular defect. Successful healing of 0-, 1-, and 3-mm defects confirms adequate stabilization of bony wounds with internal fixation with 1-mm microplates. The rat segmental mandibular critical-sized defect provides a clinically relevant testing ground for translatable mandibular tissue engineering efforts.
AB - IMPORTANCE Advances in tissue engineering offer potential alternatives to current mandibular reconstructive techniques; however, before clinical translation of this technology, a relevant animal model must be used to validate possible interventions. OBJECTIVE To establish the critical-sized segmental mandibular defect that does not heal spontaneously in the rat mandible. DESIGN AND SETTING Prospective study of mandibular defect healing in 29 Sprague-Dawley rats in an animal laboratory. INTERVENTIONS The rats underwent creation of 1 of 4 segmental mandibular defects measuring 0, 1, 3, and 5 mm. All mandibular wounds were internally fixated with 1-mm microplates and screws and allowed to heal for 12 weeks, after which the animals were killed humanely. MAIN OUTCOMES AND MEASURES Analysis with micro-computed tomography of bony union and formation graded on semiquantitative scales. RESULTS Seven animals were included in each experimental group. No 5-mm segmental defects successfully developed bony union, whereas all 0- and 1-mm defects had continuous bony growth across the original defect on micro-computed tomography. Three of the 3-mm defects had bony continuity, and 3 had no healing of the bony wound. Bone union scores were significantly lower for the 5-mm defects compared with the 0-, 1-, and 3-mm defects (P < .01). CONCLUSIONS AND RELEVANCE The rat segmental mandible model cannot heal a 5-mm segmental mandibular defect. Successful healing of 0-, 1-, and 3-mm defects confirms adequate stabilization of bony wounds with internal fixation with 1-mm microplates. The rat segmental mandibular critical-sized defect provides a clinically relevant testing ground for translatable mandibular tissue engineering efforts.
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U2 - 10.1001/jamaoto.2013.5669
DO - 10.1001/jamaoto.2013.5669
M3 - Article
C2 - 24232293
AN - SCOPUS:84892689888
SN - 2168-6181
VL - 140
SP - 58
EP - 65
JO - JAMA Otolaryngology - Head and Neck Surgery
JF - JAMA Otolaryngology - Head and Neck Surgery
IS - 1
ER -