Denervation effects on newt limb regeneration: DNA polymerase activity in vitro

Marc H. Dresden; Robb E. Moses

doi:10.1016/0012-1606(73)90363-1

Denervation effects on newt limb regeneration: DNA polymerase activity in vitro

Marc H. Dresden
, Robb E. Moses

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

DNA polymerase activity in extracts of newt (Triturus viridescens) tissues was assayed in an effort to characterize the effect of nerve resection on the regeneration of amputated limbs. Regenerating limbs display 5-20 times more polymerase activity than nonregenerating limbs. Denervation of partially regenerated limbs, which completely prevents further regeneration in vivo does not, however, affect DNA polymerase activity assayed in vitro. These results suggest that while DNA polymerase activity is responsive to the stimulus of regeneration, denervation effects are probably not directly exerted on this enzyme activity.

Original language	English (US)
Pages (from-to)	346-350
Number of pages	5
Journal	Developmental Biology
Volume	34
Issue number	2
DOIs	https://doi.org/10.1016/0012-1606(73)90363-1
State	Published - Oct 1973
Externally published	Yes

Funding

The technical assistance of Mrs. Dora Payne and Mr. David Bowersock is gratefully acknowledged. This work was supported by a contract from the U.S. Atomic Energy Commission (M.H.D.) and grants from the National Science Foundation (M.H.D.) and the National Institutes of Health (R.E.M.). R.E.M. holds a Career Development Award (GM-70,314).

Funders
Author National Science Foundation National Science Foundation National Institutes of Health National Institutes of Health National Institutes of Health National Institutes of Health National Science Foundation National Science Foundation
Author National Institutes of Health National Institutes of Health National Institutes of Health National Institutes of Health The Bev Hartig Huntington's Disease Foundation National Institutes of Health
United States Atomic Energy Commission

ASJC Scopus subject areas

Molecular Biology
Developmental Biology
Cell Biology

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title = "Denervation effects on newt limb regeneration: DNA polymerase activity in vitro",

abstract = "DNA polymerase activity in extracts of newt (Triturus viridescens) tissues was assayed in an effort to characterize the effect of nerve resection on the regeneration of amputated limbs. Regenerating limbs display 5-20 times more polymerase activity than nonregenerating limbs. Denervation of partially regenerated limbs, which completely prevents further regeneration in vivo does not, however, affect DNA polymerase activity assayed in vitro. These results suggest that while DNA polymerase activity is responsive to the stimulus of regeneration, denervation effects are probably not directly exerted on this enzyme activity.",

author = "Dresden, \{Marc H.\} and Moses, \{Robb E.\}",

note = "Funding Information: The technical assistance of Mrs. Dora Payne and Mr. David Bowersock is gratefully acknowledged. This work was supported by a contract from the U.S. Atomic Energy Commission (M.H.D.) and grants from the National Science Foundation (M.H.D.) and the National Institutes of Health (R.E.M.). R.E.M. holds a Career Development Award (GM-70,314).",

year = "1973",

month = oct,

doi = "10.1016/0012-1606(73)90363-1",

language = "English (US)",

volume = "34",

pages = "346--350",

journal = "Developmental Biology",

issn = "0012-1606",

publisher = "Elsevier Inc.",

number = "2",

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T1 - Denervation effects on newt limb regeneration

T2 - DNA polymerase activity in vitro

AU - Dresden, Marc H.

AU - Moses, Robb E.

N1 - Funding Information: The technical assistance of Mrs. Dora Payne and Mr. David Bowersock is gratefully acknowledged. This work was supported by a contract from the U.S. Atomic Energy Commission (M.H.D.) and grants from the National Science Foundation (M.H.D.) and the National Institutes of Health (R.E.M.). R.E.M. holds a Career Development Award (GM-70,314).

PY - 1973/10

Y1 - 1973/10

N2 - DNA polymerase activity in extracts of newt (Triturus viridescens) tissues was assayed in an effort to characterize the effect of nerve resection on the regeneration of amputated limbs. Regenerating limbs display 5-20 times more polymerase activity than nonregenerating limbs. Denervation of partially regenerated limbs, which completely prevents further regeneration in vivo does not, however, affect DNA polymerase activity assayed in vitro. These results suggest that while DNA polymerase activity is responsive to the stimulus of regeneration, denervation effects are probably not directly exerted on this enzyme activity.

AB - DNA polymerase activity in extracts of newt (Triturus viridescens) tissues was assayed in an effort to characterize the effect of nerve resection on the regeneration of amputated limbs. Regenerating limbs display 5-20 times more polymerase activity than nonregenerating limbs. Denervation of partially regenerated limbs, which completely prevents further regeneration in vivo does not, however, affect DNA polymerase activity assayed in vitro. These results suggest that while DNA polymerase activity is responsive to the stimulus of regeneration, denervation effects are probably not directly exerted on this enzyme activity.

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AN - SCOPUS:0015840701

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ER -

Denervation effects on newt limb regeneration: DNA polymerase activity in vitro

Abstract

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ASJC Scopus subject areas

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