TY - JOUR
T1 - Dermoscopic features associated with 3-GEP PLA
T2 - LINC00518, PRAME, and TERT expression in suspicious pigmented lesions
AU - Ludzik, Joanna
AU - Becker, Alyssa L.
AU - Latour, Emile
AU - Lee, Claudia
AU - Witkowski, Alexander
N1 - Publisher Copyright:
© 2023 The Authors. Skin Research and Technology published by John Wiley & Sons Ltd.
PY - 2023/4
Y1 - 2023/4
N2 - Utilization of dermoscopy and novel molecular triage technologies augments visual triage of pigmented skin lesions, promoting early detection of melanoma. One emerging in vivo genomic test, 3-GEP pigmented lesion assay (3-GEP PLA) aids in pigmented lesion triage by noninvasively detecting the presence of three genes associated with melanoma: LINC00518, PRAME, and TERT. The purpose of our retrospective case-control study was to identify dermoscopic features uniquely associated with the presence of LINC00518, PRAME, or TERT in the stratum corneum as determined by 3-GEP PLA testing. Images of suspicious pigmented lesions that had undergone 3-GEP PLA testing and received a definitive positive or negative result (n = 393) were evaluated for the presence of specific clinical and dermoscopic features associated with melanoma. We found that asymmetry of color was a significant predictor for PRAME expression (Odds Ratio (OR) 5.5, 95% Confidence Interval (CI) 1.6–34.5, p = 0.004), blue color and negative pigment network were significant predictors for LINC00518 expression (adjusted OR 2.7, 95% CI 1.2–5.5, p = 0.014 and adjusted OR 5.4, 95% CI 1.6–16.9, p = 0.010, respectively), and atypical polymorphous vessels present in a pigmented skin lesion were a significant predictor for TERT promoter mutations (OR 5.8, 95% CI 1.3–23.4, p = 0.022). The results presented suggest a hierarchy in the significance of these dermoscopic features and may help guide evaluation and management of pigmented skin lesions.
AB - Utilization of dermoscopy and novel molecular triage technologies augments visual triage of pigmented skin lesions, promoting early detection of melanoma. One emerging in vivo genomic test, 3-GEP pigmented lesion assay (3-GEP PLA) aids in pigmented lesion triage by noninvasively detecting the presence of three genes associated with melanoma: LINC00518, PRAME, and TERT. The purpose of our retrospective case-control study was to identify dermoscopic features uniquely associated with the presence of LINC00518, PRAME, or TERT in the stratum corneum as determined by 3-GEP PLA testing. Images of suspicious pigmented lesions that had undergone 3-GEP PLA testing and received a definitive positive or negative result (n = 393) were evaluated for the presence of specific clinical and dermoscopic features associated with melanoma. We found that asymmetry of color was a significant predictor for PRAME expression (Odds Ratio (OR) 5.5, 95% Confidence Interval (CI) 1.6–34.5, p = 0.004), blue color and negative pigment network were significant predictors for LINC00518 expression (adjusted OR 2.7, 95% CI 1.2–5.5, p = 0.014 and adjusted OR 5.4, 95% CI 1.6–16.9, p = 0.010, respectively), and atypical polymorphous vessels present in a pigmented skin lesion were a significant predictor for TERT promoter mutations (OR 5.8, 95% CI 1.3–23.4, p = 0.022). The results presented suggest a hierarchy in the significance of these dermoscopic features and may help guide evaluation and management of pigmented skin lesions.
KW - LINC00518
KW - PRAME
KW - TERT
KW - dermatoscopy
KW - dermoscopy
KW - gene expression profiling
KW - melanoma
KW - pigmented lesion assay
KW - pigmented lesions
KW - skin cancer
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U2 - 10.1111/srt.13323
DO - 10.1111/srt.13323
M3 - Article
C2 - 37083005
AN - SCOPUS:85153427308
SN - 0909-752X
VL - 29
JO - Skin Research and Technology
JF - Skin Research and Technology
IS - 4
M1 - e13323
ER -