@inbook{2575cdd69c074bd2aad022c3aa4b0492,
title = "Detecting protein ADP-ribosylation using a clickable aminooxy probe",
abstract = "ADP-ribosylation, a posttranslational modification catalyzed by a family of enzymes known as poly(ADP-ribose) polymerases (PARPs, 17 in humans), regulates diverse cellular processes. To aid in understanding the functions of ADP-ribosylation in cells, we developed a clickable aminooxy probe, AO-alkyne, which detects ADP-ribosylation of acidic amino acids. AO-alkyne can be used to detect auto-ADP-ribosylation of PARP10 in cells following Cu-catalyzed click conjugation to an azide reporter. This method can be extended to other PARP family members that catalyze ADP-ribosylation on acidic amino acids, providing a convenient and direct readout of PARP activity in cells.",
keywords = "ADP-ribosylation, Bioconjugation, Click chemistry, Oxime ligation, PARPs",
author = "Morgan, {Rory K.} and Cohen, {Michael S.}",
note = "Publisher Copyright: {\textcopyright} 2017, Springer Science+Business Media LLC.",
year = "2017",
doi = "10.1007/978-1-4939-6993-7_6",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "71--77",
booktitle = "Methods in Molecular Biology",
}