@inbook{a677122210b942b99c8f34550a86500c,
title = "Detection of post-translational modifications on MYC",
abstract = "Detection of post-translational modifications in c-Myc is an invaluable tool in assessing Myc status, particularly in cancer. However, it can be challenging to detect these modifications. The evaluation of phosphorylation status of c-Myc can also be challenging with the current commercially available phosphorylation sensitive antibodies. Here, we describe protocols for the immunoprecipitation of endogenous c-Myc to probe for phosphorylation status, as well as the detection of ubiquitination and SUMOylation on c-Myc. We will also discuss the challenges of detecting phosphorylated c-Myc in formalin-fixed paraffin-embedded tissues by immunofluorescence and describe a protocol using a new rat monoclonal antibody we have generated suitable for this purpose.",
keywords = "Immunofluorescence, Immunoprecipitation, Phosphorylation, SUMOylation, Ubiquitination, c-Myc",
author = "Daniel, {Colin J.} and Sun, {Xiao Xin} and Yingxiao Chen and Xiaoli Zhang and Dai, {Mu Shui} and Sears, {Rosalie C.}",
note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media, LLC, part of Springer Nature 2021.",
year = "2021",
doi = "10.1007/978-1-0716-1476-1_5",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "69--85",
booktitle = "Methods in Molecular Biology",
}