TY - JOUR
T1 - Determination of acrylamide in nerve tissue homogenates by electron-capture gas chromatography
AU - Poole, C. F.
AU - Sye, W. F.
AU - Zlatkis, A.
AU - Spencer, P. S.
PY - 1981/11/6
Y1 - 1981/11/6
N2 - Acrylamide in biological samples can be determined by gas chromatography with electron-capture detection after conversion to its 2,3-dibromopropionamide derivative. The derivatization is carried out in aqueous solution, plasma or tissue homogenates by ionic bromination and the reaction conditions and sample clean-up are described. The detection limit corresponds to 9.5 {star, open} 10-12 g of acrylamide on column or 8.4 {star, open} 10-9 g in the final biological extract (0.5 ml). The overall recovery of acrylamide spiked samples at the nanogram level exceeds 80%. It was found that the accumulation of free acrylamide in the science nerve region of rats intoxicated with acrylamide was less than 2-8 ppm.
AB - Acrylamide in biological samples can be determined by gas chromatography with electron-capture detection after conversion to its 2,3-dibromopropionamide derivative. The derivatization is carried out in aqueous solution, plasma or tissue homogenates by ionic bromination and the reaction conditions and sample clean-up are described. The detection limit corresponds to 9.5 {star, open} 10-12 g of acrylamide on column or 8.4 {star, open} 10-9 g in the final biological extract (0.5 ml). The overall recovery of acrylamide spiked samples at the nanogram level exceeds 80%. It was found that the accumulation of free acrylamide in the science nerve region of rats intoxicated with acrylamide was less than 2-8 ppm.
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U2 - 10.1016/S0021-9673(00)88078-4
DO - 10.1016/S0021-9673(00)88078-4
M3 - Article
C2 - 7320111
AN - SCOPUS:0019731997
SN - 0021-9673
VL - 217
SP - 239
EP - 245
JO - Journal of Chromatography A
JF - Journal of Chromatography A
IS - C
ER -