Developmentally regulated transporter in Leishmania is encoded by a family of clustered genes

David A. Stein; Bradley R. Cairns; Scott M. Landfear

doi:10.1093/nar/18.6.1549

Developmentally regulated transporter in Leishmania is encoded by a family of clustered genes

David A. Stein
, Bradley R. Cairns
, Scott M. Landfear

Molecular Microbiology and Immunology

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

We have previously cloned a gene for a developmentally regulated transport protein from the trypanosomatid protozoan Leishmania enriettil. We demonstrate here that this transporter is encoded by a single family of tandemly clustered genes containing approximately 8 copies of the 3.6 kilobase repeat unit. Transcriptional mapping defines a contiguous 3.3 kilobase region of the repeat unit that encodes the mRNA. The 5′ end of the mature mRNA contains the spliced leader or mlni-exon previously identified in kinetoplastid protozoa, while the 3′ ends of the mRNA are heterogeneous in sequence and in location of the polyadenylation site. We have identified genomic restriction fragments that flank the tandem repeat on the 5′ and 3′ sides and which may be linked to sequences required for expression of the gene family. Other species of Leishmanla also contain sequences that hybridize to the cloned L. enriettil gene at high stringency.

Original language	English (US)
Pages (from-to)	1549-1557
Number of pages	9
Journal	Nucleic acids research
Volume	18
Issue number	6
DOIs	https://doi.org/10.1093/nar/18.6.1549
State	Published - Mar 25 1990

Funding

We thank Dr. Buddy Ullman for a gift of genomic DNA from the DI700 clone of Leishmania donovani and Dr. Mary Stenzel-Poore for advice on primer extension sequencing. We also thank Dr. Margaret H. Baron and Dr. Enc Barklis for thoughtful comments on the manuscript. This work was supported by N1H grant AI25920 and by a grant from the United Nations Development Programme/World Bank/World Health Organization Special Programme for Research and Training in Tropical Diseases.

Funders	Funder number
United Nations Development Programme/World Bank/World Health Organization
National Institute of Allergy and Infectious Diseases	R01AI025920

ASJC Scopus subject areas

Genetics

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title = "Developmentally regulated transporter in Leishmania is encoded by a family of clustered genes",

abstract = "We have previously cloned a gene for a developmentally regulated transport protein from the trypanosomatid protozoan Leishmania enriettil. We demonstrate here that this transporter is encoded by a single family of tandemly clustered genes containing approximately 8 copies of the 3.6 kilobase repeat unit. Transcriptional mapping defines a contiguous 3.3 kilobase region of the repeat unit that encodes the mRNA. The 5′ end of the mature mRNA contains the spliced leader or mlni-exon previously identified in kinetoplastid protozoa, while the 3′ ends of the mRNA are heterogeneous in sequence and in location of the polyadenylation site. We have identified genomic restriction fragments that flank the tandem repeat on the 5′ and 3′ sides and which may be linked to sequences required for expression of the gene family. Other species of Leishmanla also contain sequences that hybridize to the cloned L. enriettil gene at high stringency.",

author = "Stein, \{David A.\} and Cairns, \{Bradley R.\} and Landfear, \{Scott M.\}",

note = "Funding Information: We thank Dr. Buddy Ullman for a gift of genomic DNA from the DI700 clone of Leishmania donovani and Dr. Mary Stenzel-Poore for advice on primer extension sequencing. We also thank Dr. Margaret H. Baron and Dr. Enc Barklis for thoughtful comments on the manuscript. This work was supported by N1H grant AI25920 and by a grant from the United Nations Development Programme/World Bank/World Health Organization Special Programme for Research and Training in Tropical Diseases.",

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doi = "10.1093/nar/18.6.1549",

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AU - Cairns, Bradley R.

AU - Landfear, Scott M.

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PY - 1990/3/25

Y1 - 1990/3/25

N2 - We have previously cloned a gene for a developmentally regulated transport protein from the trypanosomatid protozoan Leishmania enriettil. We demonstrate here that this transporter is encoded by a single family of tandemly clustered genes containing approximately 8 copies of the 3.6 kilobase repeat unit. Transcriptional mapping defines a contiguous 3.3 kilobase region of the repeat unit that encodes the mRNA. The 5′ end of the mature mRNA contains the spliced leader or mlni-exon previously identified in kinetoplastid protozoa, while the 3′ ends of the mRNA are heterogeneous in sequence and in location of the polyadenylation site. We have identified genomic restriction fragments that flank the tandem repeat on the 5′ and 3′ sides and which may be linked to sequences required for expression of the gene family. Other species of Leishmanla also contain sequences that hybridize to the cloned L. enriettil gene at high stringency.

AB - We have previously cloned a gene for a developmentally regulated transport protein from the trypanosomatid protozoan Leishmania enriettil. We demonstrate here that this transporter is encoded by a single family of tandemly clustered genes containing approximately 8 copies of the 3.6 kilobase repeat unit. Transcriptional mapping defines a contiguous 3.3 kilobase region of the repeat unit that encodes the mRNA. The 5′ end of the mature mRNA contains the spliced leader or mlni-exon previously identified in kinetoplastid protozoa, while the 3′ ends of the mRNA are heterogeneous in sequence and in location of the polyadenylation site. We have identified genomic restriction fragments that flank the tandem repeat on the 5′ and 3′ sides and which may be linked to sequences required for expression of the gene family. Other species of Leishmanla also contain sequences that hybridize to the cloned L. enriettil gene at high stringency.

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Developmentally regulated transporter in Leishmania is encoded by a family of clustered genes

Abstract

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