Different Chinese hamster cell lines express a G1 period for different reasons

R. Michael Liskay, Katherine E. Leonard, David M. Prescott

Research output: Contribution to journalArticlepeer-review

1 Scopus citations


Previous studies from our laboratory have shown that the absence of G1 (G1- condition) in two lines of Chinese hamster cells is dominant over the presence of G1 (G1+ condition) in a variety of intraspecific cell hybrids. G1+ mutants or variants can be isolated from G1- cells following mutagenesis and selection. These G1+ mutants fall into multiple complementation groups based on their abilities to form G1- cell hybrids with one another. This is evidence that different mutants have G1 intervals for different reasons, possibly as the result of deficiencies in functions necessary for G1- cell cycles. In this report we have used cell hybrid analysis to ask whether cells of different, naturally occurring G1+ lines of Chinese hamster are able to complement to produce G1- hybrids. We have found three complementation groups among the four G1+ cell lines examined. Therefore, these lines define three different reasons or bases for the existence of a G1 interval. These results lead us to suggest that multiple requirements must be met for these cells to start the S period, but that failure to fulfill only a single and different requirement is responsible for the presence of a G1 interval in any given cell line.

Original languageEnglish (US)
Pages (from-to)615-623
Number of pages9
JournalSomatic Cell Genetics
Issue number5
StatePublished - Sep 1979

ASJC Scopus subject areas

  • Genetics


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