Differential loss of dopamine D2 receptor mRNA isoforms during aging in Fischer-344 rats

Kalpana M. Merchant; Dorcas J. Dobie; Daniel M. Dorsa

doi:10.1016/0304-3940(93)90197-S

Differential loss of dopamine D2 receptor mRNA isoforms during aging in Fischer-344 rats

Kalpana M. Merchant, Dorcas J. Dobie, Daniel M. Dorsa

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Effects of aging on the expression of dopamine D2 receptor isoforms (D2-long and -short) in neostriatal subregions was examined by in situ hybridization histochemistry. Hybridization of a probe selective for the D2-long transcript was compared with signal generated by a probe recognizing both variants of D2 mRNA. Lateral quadrants of the neostriatum in old rats appeared to show declines in primarily the long transcripts. On the other hand, decreases in the expression of D2-short transcripts appeared to occur during aging in the ventromedial region of the striatum. These data suggest that the mechanisms involved in alternative splicing of D2 mRNA may be differentially altered in neostriatal subregions during aging.

Original language	English (US)
Pages (from-to)	163-167
Number of pages	5
Journal	Neuroscience Letters
Volume	154
Issue number	1-2
DOIs	https://doi.org/10.1016/0304-3940(93)90197-S
State	Published - May 14 1993
Externally published	Yes

Keywords

Dopamine D2 receptor mRNA
In situ hybridization
Movement deficit
Senescence

ASJC Scopus subject areas

Neuroscience(all)

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10.1016/0304-3940(93)90197-S

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@article{9720aac093534a59bcc925aeda5522ec,

title = "Differential loss of dopamine D2 receptor mRNA isoforms during aging in Fischer-344 rats",

abstract = "Effects of aging on the expression of dopamine D2 receptor isoforms (D2-long and -short) in neostriatal subregions was examined by in situ hybridization histochemistry. Hybridization of a probe selective for the D2-long transcript was compared with signal generated by a probe recognizing both variants of D2 mRNA. Lateral quadrants of the neostriatum in old rats appeared to show declines in primarily the long transcripts. On the other hand, decreases in the expression of D2-short transcripts appeared to occur during aging in the ventromedial region of the striatum. These data suggest that the mechanisms involved in alternative splicing of D2 mRNA may be differentially altered in neostriatal subregions during aging.",

keywords = "Dopamine D2 receptor mRNA, In situ hybridization, Movement deficit, Senescence",

author = "Merchant, {Kalpana M.} and Dobie, {Dorcas J.} and Dorsa, {Daniel M.}",

note = "Funding Information: We are grateful to Dr. Mark Hamblin for his assistance with cloning of the D2 receptor cDNA used in the present study for transcription of the non-discriminatory cRNA probe. We are thankful also for the technical assistance provided by RaeLee Parker and Anne Kallio-maki. This research was supported by the Scottish Rite Schizophrenia Research Program, Geriatric Academic Program Award, Department of Veterans Affairs and NS 20311.",

year = "1993",

month = may,

day = "14",

doi = "10.1016/0304-3940(93)90197-S",

language = "English (US)",

volume = "154",

pages = "163--167",

journal = "Neuroscience Letters",

issn = "0304-3940",

publisher = "Elsevier Ireland Ltd",

number = "1-2",

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TY - JOUR

T1 - Differential loss of dopamine D2 receptor mRNA isoforms during aging in Fischer-344 rats

AU - Merchant, Kalpana M.

AU - Dobie, Dorcas J.

AU - Dorsa, Daniel M.

N1 - Funding Information: We are grateful to Dr. Mark Hamblin for his assistance with cloning of the D2 receptor cDNA used in the present study for transcription of the non-discriminatory cRNA probe. We are thankful also for the technical assistance provided by RaeLee Parker and Anne Kallio-maki. This research was supported by the Scottish Rite Schizophrenia Research Program, Geriatric Academic Program Award, Department of Veterans Affairs and NS 20311.

PY - 1993/5/14

Y1 - 1993/5/14

N2 - Effects of aging on the expression of dopamine D2 receptor isoforms (D2-long and -short) in neostriatal subregions was examined by in situ hybridization histochemistry. Hybridization of a probe selective for the D2-long transcript was compared with signal generated by a probe recognizing both variants of D2 mRNA. Lateral quadrants of the neostriatum in old rats appeared to show declines in primarily the long transcripts. On the other hand, decreases in the expression of D2-short transcripts appeared to occur during aging in the ventromedial region of the striatum. These data suggest that the mechanisms involved in alternative splicing of D2 mRNA may be differentially altered in neostriatal subregions during aging.

AB - Effects of aging on the expression of dopamine D2 receptor isoforms (D2-long and -short) in neostriatal subregions was examined by in situ hybridization histochemistry. Hybridization of a probe selective for the D2-long transcript was compared with signal generated by a probe recognizing both variants of D2 mRNA. Lateral quadrants of the neostriatum in old rats appeared to show declines in primarily the long transcripts. On the other hand, decreases in the expression of D2-short transcripts appeared to occur during aging in the ventromedial region of the striatum. These data suggest that the mechanisms involved in alternative splicing of D2 mRNA may be differentially altered in neostriatal subregions during aging.

KW - Dopamine D2 receptor mRNA

KW - In situ hybridization

KW - Movement deficit

KW - Senescence

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SN - 0304-3940

VL - 154

SP - 163

EP - 167

JO - Neuroscience Letters

JF - Neuroscience Letters

IS - 1-2

ER -

Differential loss of dopamine D2 receptor mRNA isoforms during aging in Fischer-344 rats

Abstract

Keywords

ASJC Scopus subject areas

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