Direct binding of hepatitis B virus X protein and retinoid X receptor contributes to phosphoenolpyruvate carboxykinase gene transactivation

Hee Jeong Kong; Sun Hwa Hong; Min Young Lee; Han Do Kim; Jae Woon Lee; Jae Hun Cheong

doi:10.1016/S0014-5793(00)02091-3

Direct binding of hepatitis B virus X protein and retinoid X receptor contributes to phosphoenolpyruvate carboxykinase gene transactivation

Hee Jeong Kong
, Sun Hwa Hong
, Min Young Lee
, Han Do Kim
, Jae Woon Lee
, Jae Hun Cheong

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

The X gene product of the human hepatitis B virus (HBx), a major factor responsible for hepatitis and hepatocellular carcinoma, modulates transactivation by a variety of transcription factors. Herein, expression of the phosphoenolpyruvate carboxykinase (PEPCK) gene was found to be regulated transcriptionally by HBx through two distinct promoter regions. The cAMP response element (CRE)-1 site within the proximal promoter region mediated the HBx-induced transactivation of the PEPCK gene through C/EBPα and ATF-2. A retinoid X receptor (RXR) response element within the distal promoter region also contributed to the HBx-induced transactivation. Consistent with these results, HBx directly interacted with RXR, and the interaction interfaces were localized to the transactivation domain of HBx and the ligand binding domain of RXR. (C) 2000 Federation of European Biochemical Societies.

Original language	English (US)
Pages (from-to)	114-118
Number of pages	5
Journal	FEBS Letters
Volume	483
Issue number	2-3
DOIs	https://doi.org/10.1016/S0014-5793(00)02091-3
State	Published - Oct 20 2000
Externally published	Yes

Funding

We thank Dr. Seishi Murakami for the HBx expression constructs. This research was exclusively supported by the National Creative Research Initiatives Program of the Korean Ministry of Science and Technology (awarded to J.W.L.).

Funders
Ministry of Education, Science and Technology

Keywords

ATF-2
Hepatitis B virus X protein
Phosphoenolpyruvate carboxykinase
Retinoid X receptor
Transactivation

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/S0014-5793(00)02091-3

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title = "Direct binding of hepatitis B virus X protein and retinoid X receptor contributes to phosphoenolpyruvate carboxykinase gene transactivation",

abstract = "The X gene product of the human hepatitis B virus (HBx), a major factor responsible for hepatitis and hepatocellular carcinoma, modulates transactivation by a variety of transcription factors. Herein, expression of the phosphoenolpyruvate carboxykinase (PEPCK) gene was found to be regulated transcriptionally by HBx through two distinct promoter regions. The cAMP response element (CRE)-1 site within the proximal promoter region mediated the HBx-induced transactivation of the PEPCK gene through C/EBPα and ATF-2. A retinoid X receptor (RXR) response element within the distal promoter region also contributed to the HBx-induced transactivation. Consistent with these results, HBx directly interacted with RXR, and the interaction interfaces were localized to the transactivation domain of HBx and the ligand binding domain of RXR. (C) 2000 Federation of European Biochemical Societies.",

keywords = "ATF-2, Hepatitis B virus X protein, Phosphoenolpyruvate carboxykinase, Retinoid X receptor, Transactivation",

author = "Kong, \{Hee Jeong\} and Hong, \{Sun Hwa\} and Lee, \{Min Young\} and Kim, \{Han Do\} and Lee, \{Jae Woon\} and Cheong, \{Jae Hun\}",

note = "Funding Information: We thank Dr. Seishi Murakami for the HBx expression constructs. This research was exclusively supported by the National Creative Research Initiatives Program of the Korean Ministry of Science and Technology (awarded to J.W.L.).",

year = "2000",

month = oct,

day = "20",

doi = "10.1016/S0014-5793(00)02091-3",

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AU - Kong, Hee Jeong

AU - Hong, Sun Hwa

AU - Lee, Min Young

AU - Kim, Han Do

AU - Lee, Jae Woon

AU - Cheong, Jae Hun

N1 - Funding Information: We thank Dr. Seishi Murakami for the HBx expression constructs. This research was exclusively supported by the National Creative Research Initiatives Program of the Korean Ministry of Science and Technology (awarded to J.W.L.).

PY - 2000/10/20

Y1 - 2000/10/20

N2 - The X gene product of the human hepatitis B virus (HBx), a major factor responsible for hepatitis and hepatocellular carcinoma, modulates transactivation by a variety of transcription factors. Herein, expression of the phosphoenolpyruvate carboxykinase (PEPCK) gene was found to be regulated transcriptionally by HBx through two distinct promoter regions. The cAMP response element (CRE)-1 site within the proximal promoter region mediated the HBx-induced transactivation of the PEPCK gene through C/EBPα and ATF-2. A retinoid X receptor (RXR) response element within the distal promoter region also contributed to the HBx-induced transactivation. Consistent with these results, HBx directly interacted with RXR, and the interaction interfaces were localized to the transactivation domain of HBx and the ligand binding domain of RXR. (C) 2000 Federation of European Biochemical Societies.

AB - The X gene product of the human hepatitis B virus (HBx), a major factor responsible for hepatitis and hepatocellular carcinoma, modulates transactivation by a variety of transcription factors. Herein, expression of the phosphoenolpyruvate carboxykinase (PEPCK) gene was found to be regulated transcriptionally by HBx through two distinct promoter regions. The cAMP response element (CRE)-1 site within the proximal promoter region mediated the HBx-induced transactivation of the PEPCK gene through C/EBPα and ATF-2. A retinoid X receptor (RXR) response element within the distal promoter region also contributed to the HBx-induced transactivation. Consistent with these results, HBx directly interacted with RXR, and the interaction interfaces were localized to the transactivation domain of HBx and the ligand binding domain of RXR. (C) 2000 Federation of European Biochemical Societies.

KW - ATF-2

KW - Hepatitis B virus X protein

KW - Phosphoenolpyruvate carboxykinase

KW - Retinoid X receptor

KW - Transactivation

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Direct binding of hepatitis B virus X protein and retinoid X receptor contributes to phosphoenolpyruvate carboxykinase gene transactivation

Abstract

Funding

Keywords

ASJC Scopus subject areas

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