Distribution of mRNA encoding a novel opioid receptor in mouse brain

C. Saez; Q. Y. Zhou; O. Civelli; J. R. Bunzow; D. K. Grandy

doi:10.1016/0167-0115(94)90217-8

Distribution of mRNA encoding a novel opioid receptor in mouse brain

C. Saez, Q. Y. Zhou, O. Civelli, J. R. Bunzow, D. K. Grandy

Research output: Contribution to journal › Article › peer-review

Abstract

We have used degenerate oligonucleotide primers and PCR to clone a novel G protein-coupled receptor from rat striatum mRNA. This clone, r21, shares extensive sequence identity with the mouse delta (δ) opioid receptor identified in NG108-15 cells. Sequence analysis of r21 revealed that it is: 91%, 59%, 75% and 60% identical to the mouse δ opioid receptor in transmembrane (TM) domains III, IV, V and VI, respectively. An antisense riboprobe spanning TMs III - VI was prepared and hybridized in situ to mouse brain sections. The distribution of r21 mRNA in the mouse brain suggests that it may encode a kappa opioid receptor subtype.

Original language	English (US)
Pages (from-to)	S13-S14
Journal	Regulatory Peptides
Volume	53
Issue number	SUPPL. 1
DOIs	https://doi.org/10.1016/0167-0115(94)90217-8
State	Published - Feb 21 1994
Externally published	Yes

ASJC Scopus subject areas

Physiology
Biochemistry
Endocrinology
Clinical Biochemistry
Cellular and Molecular Neuroscience

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10.1016/0167-0115(94)90217-8

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title = "Distribution of mRNA encoding a novel opioid receptor in mouse brain",

abstract = "We have used degenerate oligonucleotide primers and PCR to clone a novel G protein-coupled receptor from rat striatum mRNA. This clone, r21, shares extensive sequence identity with the mouse delta (δ) opioid receptor identified in NG108-15 cells. Sequence analysis of r21 revealed that it is: 91%, 59%, 75% and 60% identical to the mouse δ opioid receptor in transmembrane (TM) domains III, IV, V and VI, respectively. An antisense riboprobe spanning TMs III - VI was prepared and hybridized in situ to mouse brain sections. The distribution of r21 mRNA in the mouse brain suggests that it may encode a kappa opioid receptor subtype.",

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AU - Saez, C.

AU - Zhou, Q. Y.

AU - Civelli, O.

AU - Bunzow, J. R.

AU - Grandy, D. K.

PY - 1994/2/21

Y1 - 1994/2/21

N2 - We have used degenerate oligonucleotide primers and PCR to clone a novel G protein-coupled receptor from rat striatum mRNA. This clone, r21, shares extensive sequence identity with the mouse delta (δ) opioid receptor identified in NG108-15 cells. Sequence analysis of r21 revealed that it is: 91%, 59%, 75% and 60% identical to the mouse δ opioid receptor in transmembrane (TM) domains III, IV, V and VI, respectively. An antisense riboprobe spanning TMs III - VI was prepared and hybridized in situ to mouse brain sections. The distribution of r21 mRNA in the mouse brain suggests that it may encode a kappa opioid receptor subtype.

AB - We have used degenerate oligonucleotide primers and PCR to clone a novel G protein-coupled receptor from rat striatum mRNA. This clone, r21, shares extensive sequence identity with the mouse delta (δ) opioid receptor identified in NG108-15 cells. Sequence analysis of r21 revealed that it is: 91%, 59%, 75% and 60% identical to the mouse δ opioid receptor in transmembrane (TM) domains III, IV, V and VI, respectively. An antisense riboprobe spanning TMs III - VI was prepared and hybridized in situ to mouse brain sections. The distribution of r21 mRNA in the mouse brain suggests that it may encode a kappa opioid receptor subtype.

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Distribution of mRNA encoding a novel opioid receptor in mouse brain

Abstract

ASJC Scopus subject areas

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