Distribution of mRNA encoding a novel opioid receptor in mouse brain

C. Saez, Q. Y. Zhou, O. Civelli, J. R. Bunzow, D. K. Grandy

Research output: Contribution to journalArticlepeer-review


We have used degenerate oligonucleotide primers and PCR to clone a novel G protein-coupled receptor from rat striatum mRNA. This clone, r21, shares extensive sequence identity with the mouse delta (δ) opioid receptor identified in NG108-15 cells. Sequence analysis of r21 revealed that it is: 91%, 59%, 75% and 60% identical to the mouse δ opioid receptor in transmembrane (TM) domains III, IV, V and VI, respectively. An antisense riboprobe spanning TMs III - VI was prepared and hybridized in situ to mouse brain sections. The distribution of r21 mRNA in the mouse brain suggests that it may encode a kappa opioid receptor subtype.

Original languageEnglish (US)
Pages (from-to)S13-S14
JournalRegulatory Peptides
Issue numberSUPPL. 1
StatePublished - Feb 21 1994
Externally publishedYes

ASJC Scopus subject areas

  • Physiology
  • Biochemistry
  • Endocrinology
  • Clinical Biochemistry
  • Cellular and Molecular Neuroscience


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