Abstract
The relationship between DNA polymerases α and δ are evaluated immunologically by monoclonal antibody specificity against DNA polymerase α and murine polyclonal antiserum against calf thymus DNA polymerase δ. DNA polymerase α and δ are found to be immunologically distinct. The structural relationship between the proliferating cell nuclear antigen (PCNA)-dependent calf DNA polymerase δ and DNA polymerase α from human and calf was analyzed by two-dimensional tryptic peptide mapping of the catalytic polypeptides. The results demonstrate that the catalytic polypeptides of the PCNA-dependent calf polymerase δ and DNA polymerase α are distinct, unrelated, and do not share any common structural determinants. The immunological and structural relationship between a recently identified PCNA-independent form of DNA polymerase δ from HeLa cells was also assessed. This PCNA-independently human polymerase δ was found to be immunologically unrelated to human polymerase α but to share some immunological and structural determinants with the PCNA-dependent calf thymus polymerase δ.
Original language | English (US) |
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Pages (from-to) | 5924-5928 |
Number of pages | 5 |
Journal | Journal of Biological Chemistry |
Volume | 264 |
Issue number | 10 |
State | Published - 1989 |
Externally published | Yes |
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Cell Biology