DNA polymerases α and δ are immunologically and structurally distinct

S. W. Wong, J. Syvaoja, C. K. Tan, K. M. Downey, A. G. So, S. Linn, T. S.F. Wang

Research output: Contribution to journalArticlepeer-review

30 Scopus citations


The relationship between DNA polymerases α and δ are evaluated immunologically by monoclonal antibody specificity against DNA polymerase α and murine polyclonal antiserum against calf thymus DNA polymerase δ. DNA polymerase α and δ are found to be immunologically distinct. The structural relationship between the proliferating cell nuclear antigen (PCNA)-dependent calf DNA polymerase δ and DNA polymerase α from human and calf was analyzed by two-dimensional tryptic peptide mapping of the catalytic polypeptides. The results demonstrate that the catalytic polypeptides of the PCNA-dependent calf polymerase δ and DNA polymerase α are distinct, unrelated, and do not share any common structural determinants. The immunological and structural relationship between a recently identified PCNA-independent form of DNA polymerase δ from HeLa cells was also assessed. This PCNA-independently human polymerase δ was found to be immunologically unrelated to human polymerase α but to share some immunological and structural determinants with the PCNA-dependent calf thymus polymerase δ.

Original languageEnglish (US)
Pages (from-to)5924-5928
Number of pages5
JournalJournal of Biological Chemistry
Issue number10
StatePublished - 1989
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology


Dive into the research topics of 'DNA polymerases α and δ are immunologically and structurally distinct'. Together they form a unique fingerprint.

Cite this