Dopamine depletion alters responses to glutamate and GABA in the rat subthalamic nucleus

Ke Zhong Shen; Steven W. Johnson

doi:10.1097/00001756-200502080-00021

Dopamine depletion alters responses to glutamate and GABA in the rat subthalamic nucleus

Ke Zhong Shen, Steven W. Johnson

Research output: Contribution to journal › Article › peer-review

47 Scopus citations

Abstract

We used whole-cell recordings to compare currents evoked by glutamate and GABA receptor agonists in subthalamic nucleus neurons located ipsilateral and contralateral to unilateral 6-hydroxydopamine (6-OHDA) injections into the substantia nigra zona compacta. The ratio of currents evoked by AMPA (0.6 μM) and NMDA (20 μM) was significantly greater in neurons recorded ipsilateral to 6-OHDA lesions compared with the ratio of currents recorded in control (contralateral) neurons. Both the GABA_A agonist isoguvacine (20 μM) and the GABA_B agonist baclofen (10 μM) evoked significantly greater outward currents in subthalamic nucleus neurons ipsilateral to the lesion compared to contralateral neurons. We conclude that chronic dopamine depletion upregulates expression of GABA receptors and shifts the functional expression of ionotropic glutamate receptor subtype from NMDA to AMPA receptors in subthalamic nucleus.

Original language	English (US)
Pages (from-to)	171-174
Number of pages	4
Journal	NeuroReport
Volume	16
Issue number	2
DOIs	https://doi.org/10.1097/00001756-200502080-00021
State	Published - Feb 8 2005
Externally published	Yes

Keywords

6-OHDA
AMPA
Baclofen
Brain slice
Isoguvacine
NMDA

ASJC Scopus subject areas

Neuroscience(all)

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10.1097/00001756-200502080-00021

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title = "Dopamine depletion alters responses to glutamate and GABA in the rat subthalamic nucleus",

abstract = "We used whole-cell recordings to compare currents evoked by glutamate and GABA receptor agonists in subthalamic nucleus neurons located ipsilateral and contralateral to unilateral 6-hydroxydopamine (6-OHDA) injections into the substantia nigra zona compacta. The ratio of currents evoked by AMPA (0.6 μM) and NMDA (20 μM) was significantly greater in neurons recorded ipsilateral to 6-OHDA lesions compared with the ratio of currents recorded in control (contralateral) neurons. Both the GABAA agonist isoguvacine (20 μM) and the GABAB agonist baclofen (10 μM) evoked significantly greater outward currents in subthalamic nucleus neurons ipsilateral to the lesion compared to contralateral neurons. We conclude that chronic dopamine depletion upregulates expression of GABA receptors and shifts the functional expression of ionotropic glutamate receptor subtype from NMDA to AMPA receptors in subthalamic nucleus.",

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AU - Johnson, Steven W.

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N2 - We used whole-cell recordings to compare currents evoked by glutamate and GABA receptor agonists in subthalamic nucleus neurons located ipsilateral and contralateral to unilateral 6-hydroxydopamine (6-OHDA) injections into the substantia nigra zona compacta. The ratio of currents evoked by AMPA (0.6 μM) and NMDA (20 μM) was significantly greater in neurons recorded ipsilateral to 6-OHDA lesions compared with the ratio of currents recorded in control (contralateral) neurons. Both the GABAA agonist isoguvacine (20 μM) and the GABAB agonist baclofen (10 μM) evoked significantly greater outward currents in subthalamic nucleus neurons ipsilateral to the lesion compared to contralateral neurons. We conclude that chronic dopamine depletion upregulates expression of GABA receptors and shifts the functional expression of ionotropic glutamate receptor subtype from NMDA to AMPA receptors in subthalamic nucleus.

AB - We used whole-cell recordings to compare currents evoked by glutamate and GABA receptor agonists in subthalamic nucleus neurons located ipsilateral and contralateral to unilateral 6-hydroxydopamine (6-OHDA) injections into the substantia nigra zona compacta. The ratio of currents evoked by AMPA (0.6 μM) and NMDA (20 μM) was significantly greater in neurons recorded ipsilateral to 6-OHDA lesions compared with the ratio of currents recorded in control (contralateral) neurons. Both the GABAA agonist isoguvacine (20 μM) and the GABAB agonist baclofen (10 μM) evoked significantly greater outward currents in subthalamic nucleus neurons ipsilateral to the lesion compared to contralateral neurons. We conclude that chronic dopamine depletion upregulates expression of GABA receptors and shifts the functional expression of ionotropic glutamate receptor subtype from NMDA to AMPA receptors in subthalamic nucleus.

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KW - AMPA

KW - Baclofen

KW - Brain slice

KW - Isoguvacine

KW - NMDA

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Dopamine depletion alters responses to glutamate and GABA in the rat subthalamic nucleus

Abstract

Keywords

ASJC Scopus subject areas

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