Abstract
An increased hepatocellular hydration state (HS) that can be induced by hypotonic stress or a high glutamine uptake modulates the transcription of given genes in liver. This could be important in the acute phase (AP) of a systemic inflammation where both HS and glutamine uptake transiently increase in liver. In HepG2 hepatoma cells cultured in conditions of hypotonic stress or a high extracellular glutamine availability, a specifically decreased expression of two human mRNAs, namely those of α1-microglobulin/bikunin precursor (AMBP) and α2-HS-glycoprotein, that are also down-regulated in liver by AP, could be seen. A functional analysis of the AMBP promoter indicated that this hypotonic stress-induced down-regulation takes place at a transcriptional level. In these experiments, the mRNA level and transcription of the glyceraldehyde-3-phosphate dehydrogenase gene that are known to be unmodified in AP did not exhibit any change. Given that hypotonic stress also up-regulates the transcription of a liver gene that is also up-regulated in AP [Meisse et al. (1998) FEBS Lett. 422, 346-348], the AP-associated increase in hepatocellular HS now appears to participate in the transcriptional control of both sets of genes that are up- or down-regulated in AP. Copyright (C) 1998 Federation of European Biochemical Societies.
Original language | English (US) |
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Pages (from-to) | 15-18 |
Number of pages | 4 |
Journal | FEBS Letters |
Volume | 433 |
Issue number | 1-2 |
DOIs | |
State | Published - Aug 14 1998 |
Externally published | Yes |
Keywords
- Acute-phase gene
- Glutamine
- Hepatoma cell
- Hydration state
- Transcriptional regulation
ASJC Scopus subject areas
- Biophysics
- Structural Biology
- Biochemistry
- Molecular Biology
- Genetics
- Cell Biology