TY - JOUR
T1 - Dynamic assembly of ribbon synapses and circuit maintenance in a vertebrate sensory system
AU - Okawa, Haruhisa
AU - Yu, Wan Qing
AU - Matti, Ulf
AU - Schwarz, Karin
AU - Odermatt, Benjamin
AU - Zhong, Haining
AU - Tsukamoto, Yoshihiko
AU - Lagnado, Leon
AU - Rieke, Fred
AU - Schmitz, Frank
AU - Wong, Rachel O.L.
N1 - Funding Information:
We thank Wong lab members for helpful discussion. This work is supported by HFSP grant (L.L., F.S., R.O.W.), NIH EY01730 (M.N.), research grants from the German Research Community DFG (SFB894, TPA7 and Schm797/8-1; F.S.), and NIH EY 17101 (R.O.W.).
Publisher Copyright:
© 2019, The Author(s).
PY - 2019/12/1
Y1 - 2019/12/1
N2 - Ribbon synapses transmit information in sensory systems, but their development is not well understood. To test the hypothesis that ribbon assembly stabilizes nascent synapses, we performed simultaneous time-lapse imaging of fluorescently-tagged ribbons in retinal cone bipolar cells (BCs) and postsynaptic densities (PSD95-FP) of retinal ganglion cells (RGCs). Ribbons and PSD95-FP clusters were more stable when these components colocalized at synapses. However, synapse density on ON-alpha RGCs was unchanged in mice lacking ribbons (ribeye knockout). Wildtype BCs make both ribbon-containing and ribbon-free synapses with these GCs even at maturity. Ribbon assembly and cone BC-RGC synapse maintenance are thus regulated independently. Despite the absence of synaptic ribbons, RGCs continued to respond robustly to light stimuli, although quantitative examination of the responses revealed reduced frequency and contrast sensitivity.
AB - Ribbon synapses transmit information in sensory systems, but their development is not well understood. To test the hypothesis that ribbon assembly stabilizes nascent synapses, we performed simultaneous time-lapse imaging of fluorescently-tagged ribbons in retinal cone bipolar cells (BCs) and postsynaptic densities (PSD95-FP) of retinal ganglion cells (RGCs). Ribbons and PSD95-FP clusters were more stable when these components colocalized at synapses. However, synapse density on ON-alpha RGCs was unchanged in mice lacking ribbons (ribeye knockout). Wildtype BCs make both ribbon-containing and ribbon-free synapses with these GCs even at maturity. Ribbon assembly and cone BC-RGC synapse maintenance are thus regulated independently. Despite the absence of synaptic ribbons, RGCs continued to respond robustly to light stimuli, although quantitative examination of the responses revealed reduced frequency and contrast sensitivity.
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U2 - 10.1038/s41467-019-10123-1
DO - 10.1038/s41467-019-10123-1
M3 - Article
C2 - 31092821
AN - SCOPUS:85065796826
SN - 2041-1723
VL - 10
JO - Nature communications
JF - Nature communications
IS - 1
M1 - 2167
ER -