Abstract
Genistein, an inhibitor of tyrosine kinase, was used to determine the possible role of tyrosine kinase in the prolactin (PRL) stimulation of milk product formation and ornithine decarboxylase (ODC) activation in cultured mouse mammary gland tissue. Genistein (10-200 μM) inhibited in a dose-response fashion the PRL stimulation of casein, lipid and lactose synthesis as well as ODC activation. Genistein, however, did not inhibit the phospholipase C, phorbol myristate acetate or cAMP effects on ODC activation. These results suggest the possible involvement of tyrosine kinase in the mechanism by which PRL expresses its effects in mammary gland tissues.
Original language | English (US) |
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Pages (from-to) | 51-55 |
Number of pages | 5 |
Journal | Molecular and Cellular Endocrinology |
Volume | 83 |
Issue number | 1 |
DOIs | |
State | Published - Jan 1992 |
Externally published | Yes |
Keywords
- Genistein
- Mammary gland tissue
- Prolactin
- Tyrosine kinase
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Endocrinology