TY - JOUR
T1 - Effect of genetic cross on the detection of quantitative trait loci and a novel approach to mapping QTLs
AU - Hitzemann, Robert
AU - Demarest, Kristin
AU - Koyner, Jay
AU - Cipp, Laura
AU - Patel, Nilay
AU - Rasmussen, Eric
AU - McCaughran, James
N1 - Funding Information:
These studies were supported in part by PHS grants AA 11043 and MH 51372, a grant from the Department of Veterans Affairs, and a grant from the Howard Hughes Medical Institute.
PY - 2000
Y1 - 2000
N2 - A genome-wide scan was conducted in two F2 intercrosses, C57BL/6J (B6) × DBA/2J (D2) and BALB/cJ (C) × LP/J (LP), for three different phenotypes: basal locomotor activity, ethanol-induced locomotor activity, and haloperidol-induced catalepsy. For basal activity, significant quantitative trait loci (QTLs, LOD ≥ 4.3) were detected on chromosomes 9 and 19 for the C × LP intercross and chromosome 1 for the B6 × D2 intercross. Significant QTLs for ethanol-induced activation were detected on chromosome 6 for the C × LP intercross, and on chromosomes 1 and 2 for the B6 × D2 intercross. For haloperidol-induced catalepsy, significant QTLs were detected on chromosome 14 (two different QTLs) in the C × LP intercross, and chromosomes 1 and 9 in the B6 × D2 intercross. These data illustrate the importance of the genetic cross for QTL detection. Finally, the data reported here, and elsewhere, are also used to demonstrate a novel approach to QTL detection and localization.
AB - A genome-wide scan was conducted in two F2 intercrosses, C57BL/6J (B6) × DBA/2J (D2) and BALB/cJ (C) × LP/J (LP), for three different phenotypes: basal locomotor activity, ethanol-induced locomotor activity, and haloperidol-induced catalepsy. For basal activity, significant quantitative trait loci (QTLs, LOD ≥ 4.3) were detected on chromosomes 9 and 19 for the C × LP intercross and chromosome 1 for the B6 × D2 intercross. Significant QTLs for ethanol-induced activation were detected on chromosome 6 for the C × LP intercross, and on chromosomes 1 and 2 for the B6 × D2 intercross. For haloperidol-induced catalepsy, significant QTLs were detected on chromosome 14 (two different QTLs) in the C × LP intercross, and chromosomes 1 and 9 in the B6 × D2 intercross. These data illustrate the importance of the genetic cross for QTL detection. Finally, the data reported here, and elsewhere, are also used to demonstrate a novel approach to QTL detection and localization.
KW - Catalepsy
KW - Ethanol
KW - Haloperidol
KW - Locomotor activity
KW - Mouse
KW - Quantitative trait loci
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U2 - 10.1016/S0091-3057(00)00421-4
DO - 10.1016/S0091-3057(00)00421-4
M3 - Article
C2 - 11166067
AN - SCOPUS:0034484840
SN - 0091-3057
VL - 67
SP - 767
EP - 772
JO - Pharmacology Biochemistry and Behavior
JF - Pharmacology Biochemistry and Behavior
IS - 4
ER -