Effects of ovarian source, patient age, and menstrual cycle phase on in vitro maturation of immature human oocytes

Keith S. Whitacre; David B. Seifer; Chad I. Friedman; Serdar Coskun; Elizabeth A. Kennard; Moon H. Kim; Baha M. Alak

doi:10.1016/S0015-0282(98)00339-2

Effects of ovarian source, patient age, and menstrual cycle phase on in vitro maturation of immature human oocytes

Keith S. Whitacre
, David B. Seifer
, Chad I. Friedman
, Serdar Coskun
, Elizabeth A. Kennard
, Moon H. Kim
, Baha M. Alak

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

Objective: To determine the efficiency of in vitro maturation, expressed by nuclear maturation, of oocytes aspirated during gynecologic surgeries or collected from excised ovaries. To assess the effect of patient age and cycle phase at collection on the oocyte's ability to mature in vitro. To examine the time course of oocyte maturation in vitro. Design: Nuclear maturation based on patient criteria compared. Setting: University-based IVF program and research center. Patient(s): Consented patients undergoing gynecologic surgeries or patients undergoing oophorectomy. Intervention(s): Oocytes were maintained in culture for 48 hours and evaluated for maturation. Main Outcome Measure(s): Nuclear maturation evaluated as germinal vesicle breakdown (GVBD) or progression to the metaphase II (MII) stage. Result(s): A significantly higher percentage of oocytes collected during the follicular phase of the menstrual cycle underwent GVBD than did oocytes collected during the luteal phase (60% versus 48%, respectively). The percentage of oocytes reaching the MII stage, from these two groups, was not different. No statistically significant differences in maturation were observed in oocytes from different ovarian sources or from patients >40 or <40 years of age. Conclusion(s): These data suggest that oocytes collected during the follicular phase are more likely to undergo GVBD than oocytes collected during the luteal phase. In this study, ovarian source, age, or cycle phase did not influence the final meiotic maturation of oocytes to metaphase II.

Original language	English (US)
Pages (from-to)	1015-1021
Number of pages	7
Journal	Fertility and sterility
Volume	70
Issue number	6
DOIs	https://doi.org/10.1016/S0015-0282(98)00339-2
State	Published - Dec 1998
Externally published	Yes

Funding

Oocyte cumulus complexes (n = 115) also were obtained from the excised ovaries of 22 women undergoing oophorectomy. The age range of the patients was from 31 to 53 years. Common indications for surgery included uterine leiomyoma, abnormal uterine bleeding, uterine cancer, endometriosis, and pelvic pain. Ovarian specimens were received from the Cooperative Human Tissue Network, which is funded by the National Cancer Institute. Other investigators may have received samples from the same tissues. The menstrual cycle phase of the oophorectomy patients was determined by histologic staging of the uterine endometrium.

Funders	Funder number
National Institute of Aging	R29AG015425
National Institute of Health-National Cancer Institute

Keywords

Germinal vesicle breakdown (GVBD)
Human oocyte
Menstrual cycle phase
Metaphase II (MII)
Oocyte maturation

ASJC Scopus subject areas

Reproductive Medicine
Obstetrics and Gynecology

Access to Document

10.1016/S0015-0282(98)00339-2

Cite this

@article{0a300d4703cb48fab92e34c864ad5a0b,

title = "Effects of ovarian source, patient age, and menstrual cycle phase on in vitro maturation of immature human oocytes",

abstract = "Objective: To determine the efficiency of in vitro maturation, expressed by nuclear maturation, of oocytes aspirated during gynecologic surgeries or collected from excised ovaries. To assess the effect of patient age and cycle phase at collection on the oocyte's ability to mature in vitro. To examine the time course of oocyte maturation in vitro. Design: Nuclear maturation based on patient criteria compared. Setting: University-based IVF program and research center. Patient(s): Consented patients undergoing gynecologic surgeries or patients undergoing oophorectomy. Intervention(s): Oocytes were maintained in culture for 48 hours and evaluated for maturation. Main Outcome Measure(s): Nuclear maturation evaluated as germinal vesicle breakdown (GVBD) or progression to the metaphase II (MII) stage. Result(s): A significantly higher percentage of oocytes collected during the follicular phase of the menstrual cycle underwent GVBD than did oocytes collected during the luteal phase (60\% versus 48\%, respectively). The percentage of oocytes reaching the MII stage, from these two groups, was not different. No statistically significant differences in maturation were observed in oocytes from different ovarian sources or from patients >40 or <40 years of age. Conclusion(s): These data suggest that oocytes collected during the follicular phase are more likely to undergo GVBD than oocytes collected during the luteal phase. In this study, ovarian source, age, or cycle phase did not influence the final meiotic maturation of oocytes to metaphase II.",

keywords = "Germinal vesicle breakdown (GVBD), Human oocyte, Menstrual cycle phase, Metaphase II (MII), Oocyte maturation",

author = "Whitacre, \{Keith S.\} and Seifer, \{David B.\} and Friedman, \{Chad I.\} and Serdar Coskun and Kennard, \{Elizabeth A.\} and Kim, \{Moon H.\} and Alak, \{Baha M.\}",

note = "Funding Information: Oocyte cumulus complexes (n = 115) also were obtained from the excised ovaries of 22 women undergoing oophorectomy. The age range of the patients was from 31 to 53 years. Common indications for surgery included uterine leiomyoma, abnormal uterine bleeding, uterine cancer, endometriosis, and pelvic pain. Ovarian specimens were received from the Cooperative Human Tissue Network, which is funded by the National Cancer Institute. Other investigators may have received samples from the same tissues. The menstrual cycle phase of the oophorectomy patients was determined by histologic staging of the uterine endometrium. ",

year = "1998",

month = dec,

doi = "10.1016/S0015-0282(98)00339-2",

language = "English (US)",

volume = "70",

pages = "1015--1021",

journal = "Fertility and sterility",

issn = "0015-0282",

publisher = "Elsevier Inc.",

number = "6",

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TY - JOUR

T1 - Effects of ovarian source, patient age, and menstrual cycle phase on in vitro maturation of immature human oocytes

AU - Whitacre, Keith S.

AU - Seifer, David B.

AU - Friedman, Chad I.

AU - Coskun, Serdar

AU - Kennard, Elizabeth A.

AU - Kim, Moon H.

AU - Alak, Baha M.

N1 - Funding Information: Oocyte cumulus complexes (n = 115) also were obtained from the excised ovaries of 22 women undergoing oophorectomy. The age range of the patients was from 31 to 53 years. Common indications for surgery included uterine leiomyoma, abnormal uterine bleeding, uterine cancer, endometriosis, and pelvic pain. Ovarian specimens were received from the Cooperative Human Tissue Network, which is funded by the National Cancer Institute. Other investigators may have received samples from the same tissues. The menstrual cycle phase of the oophorectomy patients was determined by histologic staging of the uterine endometrium.

PY - 1998/12

Y1 - 1998/12

N2 - Objective: To determine the efficiency of in vitro maturation, expressed by nuclear maturation, of oocytes aspirated during gynecologic surgeries or collected from excised ovaries. To assess the effect of patient age and cycle phase at collection on the oocyte's ability to mature in vitro. To examine the time course of oocyte maturation in vitro. Design: Nuclear maturation based on patient criteria compared. Setting: University-based IVF program and research center. Patient(s): Consented patients undergoing gynecologic surgeries or patients undergoing oophorectomy. Intervention(s): Oocytes were maintained in culture for 48 hours and evaluated for maturation. Main Outcome Measure(s): Nuclear maturation evaluated as germinal vesicle breakdown (GVBD) or progression to the metaphase II (MII) stage. Result(s): A significantly higher percentage of oocytes collected during the follicular phase of the menstrual cycle underwent GVBD than did oocytes collected during the luteal phase (60% versus 48%, respectively). The percentage of oocytes reaching the MII stage, from these two groups, was not different. No statistically significant differences in maturation were observed in oocytes from different ovarian sources or from patients >40 or <40 years of age. Conclusion(s): These data suggest that oocytes collected during the follicular phase are more likely to undergo GVBD than oocytes collected during the luteal phase. In this study, ovarian source, age, or cycle phase did not influence the final meiotic maturation of oocytes to metaphase II.

AB - Objective: To determine the efficiency of in vitro maturation, expressed by nuclear maturation, of oocytes aspirated during gynecologic surgeries or collected from excised ovaries. To assess the effect of patient age and cycle phase at collection on the oocyte's ability to mature in vitro. To examine the time course of oocyte maturation in vitro. Design: Nuclear maturation based on patient criteria compared. Setting: University-based IVF program and research center. Patient(s): Consented patients undergoing gynecologic surgeries or patients undergoing oophorectomy. Intervention(s): Oocytes were maintained in culture for 48 hours and evaluated for maturation. Main Outcome Measure(s): Nuclear maturation evaluated as germinal vesicle breakdown (GVBD) or progression to the metaphase II (MII) stage. Result(s): A significantly higher percentage of oocytes collected during the follicular phase of the menstrual cycle underwent GVBD than did oocytes collected during the luteal phase (60% versus 48%, respectively). The percentage of oocytes reaching the MII stage, from these two groups, was not different. No statistically significant differences in maturation were observed in oocytes from different ovarian sources or from patients >40 or <40 years of age. Conclusion(s): These data suggest that oocytes collected during the follicular phase are more likely to undergo GVBD than oocytes collected during the luteal phase. In this study, ovarian source, age, or cycle phase did not influence the final meiotic maturation of oocytes to metaphase II.

KW - Germinal vesicle breakdown (GVBD)

KW - Human oocyte

KW - Menstrual cycle phase

KW - Metaphase II (MII)

KW - Oocyte maturation

UR - https://www.scopus.com/pages/publications/0032401004

UR - https://www.scopus.com/pages/publications/0032401004#tab=citedBy

U2 - 10.1016/S0015-0282(98)00339-2

DO - 10.1016/S0015-0282(98)00339-2

M3 - Article

C2 - 9848288

AN - SCOPUS:0032401004

SN - 0015-0282

VL - 70

SP - 1015

EP - 1021

JO - Fertility and sterility

JF - Fertility and sterility

IS - 6

ER -

Effects of ovarian source, patient age, and menstrual cycle phase on in vitro maturation of immature human oocytes

Abstract

Funding

Keywords

ASJC Scopus subject areas

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