Effects of retinoic acid treatment on release of arachidonic acid by chondrogenic cells in response to ionophore A23187

Paul O. Moon; Kenneth P. Chepenik; Devendra M. Kochhar

doi:10.1016/0024-3205(86)90556-4

Effects of retinoic acid treatment on release of arachidonic acid by chondrogenic cells in response to ionophore A23187

Paul O. Moon, Kenneth P. Chepenik, Devendra M. Kochhar

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Chondrogenic differentiation in mouse limb bud mesenchymal cells cultured at high density was suppressed by supplementation of the medium with retinoic acid in a dose-dependent fashion. Cells prelabeled with (³H) arachidonic acid were treated with 0.3 ug/ml retinoic acid. Treatment with retinoic acid increased the (³H) fatty acid in the triglyceride fraction. Furthermore, treatment with retinoic acid enhanced the release of (³H) fatty acid upon stimulation of these cells with the divalent ionophore A23187. These data permit the suggestion that there may be a correlation between altered lipid metabolism and retinoic acid's ability to disrupt chondrogenic differentiation.

Original language	English (US)
Pages (from-to)	1445-1450
Number of pages	6
Journal	Life Sciences
Volume	38
Issue number	16
DOIs	https://doi.org/10.1016/0024-3205(86)90556-4
State	Published - Apr 21 1986
Externally published	Yes

ASJC Scopus subject areas

General Pharmacology, Toxicology and Pharmaceutics
General Biochemistry, Genetics and Molecular Biology

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10.1016/0024-3205(86)90556-4

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title = "Effects of retinoic acid treatment on release of arachidonic acid by chondrogenic cells in response to ionophore A23187",

abstract = "Chondrogenic differentiation in mouse limb bud mesenchymal cells cultured at high density was suppressed by supplementation of the medium with retinoic acid in a dose-dependent fashion. Cells prelabeled with (3H) arachidonic acid were treated with 0.3 ug/ml retinoic acid. Treatment with retinoic acid increased the (3H) fatty acid in the triglyceride fraction. Furthermore, treatment with retinoic acid enhanced the release of (3H) fatty acid upon stimulation of these cells with the divalent ionophore A23187. These data permit the suggestion that there may be a correlation between altered lipid metabolism and retinoic acid's ability to disrupt chondrogenic differentiation.",

author = "Moon, {Paul O.} and Chepenik, {Kenneth P.} and Kochhar, {Devendra M.}",

note = "Funding Information: This research has been supported by NIH grants 5ROIHDI0935-06 and 5T35HL07497.",

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T1 - Effects of retinoic acid treatment on release of arachidonic acid by chondrogenic cells in response to ionophore A23187

AU - Moon, Paul O.

AU - Chepenik, Kenneth P.

AU - Kochhar, Devendra M.

N1 - Funding Information: This research has been supported by NIH grants 5ROIHDI0935-06 and 5T35HL07497.

PY - 1986/4/21

Y1 - 1986/4/21

N2 - Chondrogenic differentiation in mouse limb bud mesenchymal cells cultured at high density was suppressed by supplementation of the medium with retinoic acid in a dose-dependent fashion. Cells prelabeled with (3H) arachidonic acid were treated with 0.3 ug/ml retinoic acid. Treatment with retinoic acid increased the (3H) fatty acid in the triglyceride fraction. Furthermore, treatment with retinoic acid enhanced the release of (3H) fatty acid upon stimulation of these cells with the divalent ionophore A23187. These data permit the suggestion that there may be a correlation between altered lipid metabolism and retinoic acid's ability to disrupt chondrogenic differentiation.

AB - Chondrogenic differentiation in mouse limb bud mesenchymal cells cultured at high density was suppressed by supplementation of the medium with retinoic acid in a dose-dependent fashion. Cells prelabeled with (3H) arachidonic acid were treated with 0.3 ug/ml retinoic acid. Treatment with retinoic acid increased the (3H) fatty acid in the triglyceride fraction. Furthermore, treatment with retinoic acid enhanced the release of (3H) fatty acid upon stimulation of these cells with the divalent ionophore A23187. These data permit the suggestion that there may be a correlation between altered lipid metabolism and retinoic acid's ability to disrupt chondrogenic differentiation.

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Effects of retinoic acid treatment on release of arachidonic acid by chondrogenic cells in response to ionophore A23187

Abstract

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