Abstract
Electron-cytochemical localization of alkaline phosphatase activity was performed on G cells of Necturus maculosus antral mucosa. Alkaline phosphatase activity was localized to the nuclear membrane, the Golgi/endoplasmic reticulum, and the limiting membranes of G cell peptide-secretion vesicles. There was no specific localization of alkaline phosphatase activity to the plasma membrane. Treatment of the tissues with levamisole (an alkaline phosphatase inhibitor) did not markedly reduce the specific alkaline phosphatase activity. Specific lead deposition was reduced by removal of the substrate from the reaction mixture. The results from this study on N. maculosus G cells demonstrate that alkaline phosphatase activity can be found in a non-mammalian gastric endocrine cell and that specific activity was localized primarily to those intracellular structures involved with protein biosynthesis.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 555-561 |
| Number of pages | 7 |
| Journal | Cell and tissue research |
| Volume | 258 |
| Issue number | 3 |
| DOIs | |
| State | Published - Dec 1989 |
| Externally published | Yes |
Keywords
- Alkaline phosphatase
- Cytochemistry
- Endoplasmic reticulum, smooth
- G cell
- Gastrin
- Golgi complex
- Necturus maculosus (Urodela)
ASJC Scopus subject areas
- Pathology and Forensic Medicine
- Histology
- Cell Biology