TY - JOUR
T1 - Enantioselective measurement of the Candida metabolite d-arabinitol in human serum using multi-dimensional gas chromatography and a new chiral phase
AU - Wong, Brian
AU - Castellanos, Miguel
N1 - Funding Information:
Choral-NEB columns used m these studies, Dr Rouse for her valuable advice about using the columns and Duane Sigmund, M D for allowing us to study his patient This work was supported by Grant AI-23938 from the National Institute of Allergy and Infectious Diseases
PY - 1989
Y1 - 1989
N2 - A multi-dimensional gas chromatographic method was developed to measure the Candida metabolite d-arabinitol enantioselectively in human serum. The heptafluorobutyrate derivatives of d-arabinitol, l-arabinitol and ribitol (internal standard) were separated from other serum constituents with a 60 m × 0.32 mm fused-silica SPB-5 precolumn, and (after intermediate cold trapping) they were separated from each other with a 25 m × 0.25 mm fused-silica column coated with a new bonded chiral phase. Replicate analyses of spiked human sera showed that d-arabinitol could be quantified accurately and precisely. The d- and l-arabinitol concentrations in 24 normal adult sera were 0.20 ± 0.053 and 0.11 ± 0.040 μ/ml, respectively, and the d- and l-arabinitol/creatinine ratios were 0.023 ± 0.011 and 0.012 ± 0.0051, respectively (mean ± S.D.). In a patient with Candida albicans fungemia, the d-arabinitol/creatinine ratios rose early during infection and fell with successful treatment, whereas l-arabinitol/creatinine ratios did not change significantly. This enantioselective analytical method is more practical than earlier ones; it should facilitate further investigation of d-arabinitol as a diagnostic marker for candidiasis.
AB - A multi-dimensional gas chromatographic method was developed to measure the Candida metabolite d-arabinitol enantioselectively in human serum. The heptafluorobutyrate derivatives of d-arabinitol, l-arabinitol and ribitol (internal standard) were separated from other serum constituents with a 60 m × 0.32 mm fused-silica SPB-5 precolumn, and (after intermediate cold trapping) they were separated from each other with a 25 m × 0.25 mm fused-silica column coated with a new bonded chiral phase. Replicate analyses of spiked human sera showed that d-arabinitol could be quantified accurately and precisely. The d- and l-arabinitol concentrations in 24 normal adult sera were 0.20 ± 0.053 and 0.11 ± 0.040 μ/ml, respectively, and the d- and l-arabinitol/creatinine ratios were 0.023 ± 0.011 and 0.012 ± 0.0051, respectively (mean ± S.D.). In a patient with Candida albicans fungemia, the d-arabinitol/creatinine ratios rose early during infection and fell with successful treatment, whereas l-arabinitol/creatinine ratios did not change significantly. This enantioselective analytical method is more practical than earlier ones; it should facilitate further investigation of d-arabinitol as a diagnostic marker for candidiasis.
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U2 - 10.1016/S0378-4347(00)82606-7
DO - 10.1016/S0378-4347(00)82606-7
M3 - Article
C2 - 2613805
AN - SCOPUS:0024466557
SN - 0378-4347
VL - 495
SP - 21
EP - 30
JO - Journal of Chromatography B: Biomedical Sciences and Applications
JF - Journal of Chromatography B: Biomedical Sciences and Applications
IS - C
ER -