Encephalomyocarditis (EMC) virus infection in PC12 and C6 cells

Weiping Su, Aito Ueno-Yamanouchi, Hiroyuki Nakayama, Kunio Doi

Research output: Contribution to journalArticlepeer-review

1 Scopus citations


PC12 cells derived from rat pheochromocytoma and C6 cells derived from rat glioma were infected with 0.3 plaque forming units (PFU)/cell of the D variant of encephalomyocarditis virus (EMC-D), after pretreatment with or without nerve growth factor (NGF). The virus titres in medium and cells were investigated at 67, 12, 24, 48 and 72h post infection (HPI), and histopathology and viral antigens in cells were examined at 24 and 48 HPI, respectively. As a result, neither viral replication nor light and election microscopic changes were observed in PC12 cell cultures without NGF- pretreatment. On the contrary, in PC12 cell cultures with NGF-pretreatment, the virus titre prominently increased at 12 HPI, and peaked at 48 HPI. In addition, distinct histological and ultrastructural changes with vital antigens in cells were observed. C6 cells showed similar morphology and susceptibility to EMC-D-infection irrespective of NGF-pretreatment. Namely, the virus titres in C6 cell cultures increased slightly and vital antigens were found in a small number of C6 cells, but there were no evident histological and ultrastructural changes. These results suggest that PC12 cells pretreated with NGF and C6 cells are susceptible to EMC-D infection in vitro.

Original languageEnglish (US)
Pages (from-to)411-416
Number of pages6
JournalInternational Journal of Experimental Pathology
Issue number6
StatePublished - 1998
Externally publishedYes


  • C6 cell
  • Encephalomyocarditis virus
  • PC12 cell
  • Susceptibility

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Molecular Biology
  • Cell Biology


Dive into the research topics of 'Encephalomyocarditis (EMC) virus infection in PC12 and C6 cells'. Together they form a unique fingerprint.

Cite this