TY - JOUR
T1 - Equivalence of human and bovine dentin matrix molecules for dental pulp regeneration
T2 - proteomic analysis and biological function
AU - Horsophonphong, Sivaporn
AU - Sercia, Ashley
AU - França, Cristiane M.
AU - Tahayeri, Anthony
AU - Reddy, Ashok P.
AU - Wilmarth, Phillip A.
AU - Surarit, Rudee
AU - Smith, Anthony J.
AU - Ferracane, Jack L.
AU - Bertassoni, Luiz E.
N1 - Funding Information:
This project was supported by funding from the National Institute of Dental and Craniofacial Research (R01DE026170 and 3R01DE026170-03S1 to LEB), the OHSU-Mahidol University cooperation agreement (to SH), theOregon Clinical & Translational Research Institute (OCTRI) - Biomedical Innovation Program (BIP), the Innovation in Oral Care Awards sponsored by GlaxoSmithKline (GSK), International Association for Dental Research (IADR), the Michigan-Pittsburgh-Wyss Resource Center – Regenerative Medicine Resource Center (MPW-RM), the OHSU Fellowship for Diversity and Inclusion in Research (OHSU-OFDIR to CMF). Mass spectrometric analysis was performed by the OHSU Proteomics Shared Resource with partial support from NIH core grants P30EY010572 and P30CA069533, and S10OD012246. Thailand Research Fund through the Royal Golden Jubilee Ph.D. Program (Grant No. PHD/0060/2557) to S Horsophonphong and R Surarit.
Funding Information:
This project was supported by funding from the National Institute of Dental and Craniofacial Research ( R01DE026170 and 3R01DE026170-03S1 to LEB), the OHSU-Mahidol University cooperation agreement (to SH), the Oregon Clinical & Translational Research Institute (OCTRI) - Biomedical Innovation Program (BIP) , the Innovation in Oral Care Awards sponsored by GlaxoSmithKline (GSK) , International Association for Dental Research (IADR) , the Michigan-Pittsburgh-Wyss Resource Center – Regenerative Medicine Resource Center (MPW-RM) , the OHSU Fellowship for Diversity and Inclusion in Research (OHSU-OFDIR to CMF) . Mass spectrometric analysis was performed by the OHSU Proteomics Shared Resource with partial support from NIH core grants P30EY010572 and P30CA069533 , and S10OD012246 . Thailand Research Fund through the Royal Golden Jubilee Ph.D. Program (Grant No. PHD/0060/2557 ) to S Horsophonphong and R Surarit.
Publisher Copyright:
© 2020 Elsevier Ltd
PY - 2020/11
Y1 - 2020/11
N2 - Objective: To compare proteomics and biological function of human dentin matrix molecules (hDMMs) and bovine dentin matrix molecules (bDMMs). Design: Dentin powder from human or bovine teeth (n = 4) was demineralized in 10% (v/v) ethylenediaminetetraacetic acid for 7 days. The extracts were dialyzed, lyophilized and proteins were characterized using liquid chromatography-tandem mass spectrometry and shotgun proteomic analysis. To study biological function, mouse-derived undifferentiated dental pulp cells (OD21) were treated with 0.01, 0.1 or 1 μg/mL of hDMMs or bDMMs and proliferation was measured after 24 hours and 48 hours using 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Cell migration was assessed after 24 hours using a Boyden chamber. Alizarin Red S staining was used to evaluate mineral formation. Results: There were 307 proteins identified, of which 93 proteins were common to both species. Gene Ontology functional analysis demonstrated similar pattern of biological process in both species which consisted mainly of tissue development and biomineralization. hDMMs and bDMMs both enhanced cell proliferation. After 24 hours, all concentrations of bDMMs promoted cell proliferation (p ≤ 0.05), while hDMMs did not affect proliferation. After 48 hours, groups with 1μg/mL of bDMMs and 0.01μg/mL of hDMMs had increased cell proliferation compared to control (p ≤ 0.0001). All concentrations of hDMMs and bDMMs enhanced cell migration and mineralization (p ≤ 0.0001). Conclusion: bDMMs has similar biological functions as hDMMs. Moreover, bDMMs stimulated cell proliferation, migration and differentiation similar to hDMMs.
AB - Objective: To compare proteomics and biological function of human dentin matrix molecules (hDMMs) and bovine dentin matrix molecules (bDMMs). Design: Dentin powder from human or bovine teeth (n = 4) was demineralized in 10% (v/v) ethylenediaminetetraacetic acid for 7 days. The extracts were dialyzed, lyophilized and proteins were characterized using liquid chromatography-tandem mass spectrometry and shotgun proteomic analysis. To study biological function, mouse-derived undifferentiated dental pulp cells (OD21) were treated with 0.01, 0.1 or 1 μg/mL of hDMMs or bDMMs and proliferation was measured after 24 hours and 48 hours using 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Cell migration was assessed after 24 hours using a Boyden chamber. Alizarin Red S staining was used to evaluate mineral formation. Results: There were 307 proteins identified, of which 93 proteins were common to both species. Gene Ontology functional analysis demonstrated similar pattern of biological process in both species which consisted mainly of tissue development and biomineralization. hDMMs and bDMMs both enhanced cell proliferation. After 24 hours, all concentrations of bDMMs promoted cell proliferation (p ≤ 0.05), while hDMMs did not affect proliferation. After 48 hours, groups with 1μg/mL of bDMMs and 0.01μg/mL of hDMMs had increased cell proliferation compared to control (p ≤ 0.0001). All concentrations of hDMMs and bDMMs enhanced cell migration and mineralization (p ≤ 0.0001). Conclusion: bDMMs has similar biological functions as hDMMs. Moreover, bDMMs stimulated cell proliferation, migration and differentiation similar to hDMMs.
KW - cell differentiation
KW - dentin
KW - dentin matrix components
KW - growth factors
KW - proteomics
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U2 - 10.1016/j.archoralbio.2020.104888
DO - 10.1016/j.archoralbio.2020.104888
M3 - Article
C2 - 32932150
AN - SCOPUS:85090573803
SN - 0003-9969
VL - 119
JO - Archives of Oral Biology
JF - Archives of Oral Biology
M1 - 104888
ER -