Expression and regulation of mPer1 in immortalized GnRH neurons

J. Olcese; R. Domagalski; A. Bednorz; D. R. Weaver; H. F. Urbanski; S. Reuss; R. Middendorff

doi:10.1097/00001756-200303240-00018

Expression and regulation of mPer1 in immortalized GnRH neurons

J. Olcese, R. Domagalski, A. Bednorz, D. R. Weaver, H. F. Urbanski, S. Reuss, R. Middendorff

Oregon National Primate Research Center

Research output: Contribution to journal › Article › peer-review

33 Scopus citations

Abstract

Hypothalamic GnRH (gonadotropin-releasing hormone) neurons play a critical role in the initiation and maintenance of reproduction competence. Using the mouse GnRH neuronal cell line, GT1-7, we have characterized the expression of the gene mPer1, a recognized key element of the mammalian circadian clockwork. Both mPer1 transcripts and the 136 kDa m PER1 gene product could be detected in these cells. Immunocytochemical analysis also confirmed expression of mPER1 both in vitro and in vivo in GnRH neurons. Activation of cyclic AMP signalling pathways in vitro elevated GnRH secretion as well as mPer1 expression and nuclear mPER1 immunoreactivity. As mPER1 is known to feedback on transcriptional activities in many cell models, the data presented here point to a role for mPER1 in the regulation of gene expression in GnRH neurons, and thus in the control of neuroendocrine activities.

Original language	English (US)
Pages (from-to)	613-618
Number of pages	6
Journal	NeuroReport
Volume	14
Issue number	4
DOIs	https://doi.org/10.1097/00001756-200303240-00018
State	Published - Mar 2003

Keywords

Clock gene expression
GnRH neurons
cAMP
mPer1

ASJC Scopus subject areas

Neuroscience(all)

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10.1097/00001756-200303240-00018

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title = "Expression and regulation of mPer1 in immortalized GnRH neurons",

abstract = "Hypothalamic GnRH (gonadotropin-releasing hormone) neurons play a critical role in the initiation and maintenance of reproduction competence. Using the mouse GnRH neuronal cell line, GT1-7, we have characterized the expression of the gene mPer1, a recognized key element of the mammalian circadian clockwork. Both mPer1 transcripts and the 136 kDa m PER1 gene product could be detected in these cells. Immunocytochemical analysis also confirmed expression of mPER1 both in vitro and in vivo in GnRH neurons. Activation of cyclic AMP signalling pathways in vitro elevated GnRH secretion as well as mPer1 expression and nuclear mPER1 immunoreactivity. As mPER1 is known to feedback on transcriptional activities in many cell models, the data presented here point to a role for mPER1 in the regulation of gene expression in GnRH neurons, and thus in the control of neuroendocrine activities.",

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AU - Olcese, J.

AU - Domagalski, R.

AU - Bednorz, A.

AU - Weaver, D. R.

AU - Urbanski, H. F.

AU - Reuss, S.

AU - Middendorff, R.

PY - 2003/3

Y1 - 2003/3

N2 - Hypothalamic GnRH (gonadotropin-releasing hormone) neurons play a critical role in the initiation and maintenance of reproduction competence. Using the mouse GnRH neuronal cell line, GT1-7, we have characterized the expression of the gene mPer1, a recognized key element of the mammalian circadian clockwork. Both mPer1 transcripts and the 136 kDa m PER1 gene product could be detected in these cells. Immunocytochemical analysis also confirmed expression of mPER1 both in vitro and in vivo in GnRH neurons. Activation of cyclic AMP signalling pathways in vitro elevated GnRH secretion as well as mPer1 expression and nuclear mPER1 immunoreactivity. As mPER1 is known to feedback on transcriptional activities in many cell models, the data presented here point to a role for mPER1 in the regulation of gene expression in GnRH neurons, and thus in the control of neuroendocrine activities.

AB - Hypothalamic GnRH (gonadotropin-releasing hormone) neurons play a critical role in the initiation and maintenance of reproduction competence. Using the mouse GnRH neuronal cell line, GT1-7, we have characterized the expression of the gene mPer1, a recognized key element of the mammalian circadian clockwork. Both mPer1 transcripts and the 136 kDa m PER1 gene product could be detected in these cells. Immunocytochemical analysis also confirmed expression of mPER1 both in vitro and in vivo in GnRH neurons. Activation of cyclic AMP signalling pathways in vitro elevated GnRH secretion as well as mPer1 expression and nuclear mPER1 immunoreactivity. As mPER1 is known to feedback on transcriptional activities in many cell models, the data presented here point to a role for mPER1 in the regulation of gene expression in GnRH neurons, and thus in the control of neuroendocrine activities.

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KW - GnRH neurons

KW - cAMP

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Expression and regulation of mPer1 in immortalized GnRH neurons

Abstract

Keywords

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