Expression of mRNA and proteins for GnRH I and II and their receptors in primate corpus luteum during menstrual cycle

Nilkanta Chakrabarti, Thenmozhi Subbarao, Anamika Sengupta, Fuhua Xu, Richard L. Stouffer, Rajagopala Sridaran

    Research output: Contribution to journalArticlepeer-review

    12 Scopus citations


    The differential expression of mRNA and protein of GnRH I, II and their receptors (RI and RII) in the monkey corpus luteum (CL) were measured during different stages of the luteal phase of the menstrual cycle as an initial step towards considering the role and regulation of GnRH (I and II) system during luteinization and luteolysis in primates. RT-PCR confirmed the sequence identity of PCR products and real time PCR quantified specific mRNA expressions. Proteins were localized by immunohistochemistry (IHC). Changes in mRNA expression patterns of GnRH I and II (increased) and GnRH RII (decreased) were maximal at mid-late to late stages, that is, at CL regression, where as GnRH RI was low during the entire luteal phase. However, RT-PCR and IHC studies confirmed the presence of GnRH RI at both mRNA and protein levels, respectively. IHC results showed the presence of GnRH I, II and their receptors in steroidogenic cells (granulose-luteal cells and thecal-luteal cells) across the luteal phase. Hence, GnRH I and II systems may have a role on both luteinization (from early to mid stages of CL) and luteolysis (from mid-late to very-late stages of CL). These novel findings suggest that monkey luteal GnRH system may have a role in fertility regulation in paracrine and/or autocrine manner.

    Original languageEnglish (US)
    Pages (from-to)1567-1577
    Number of pages11
    JournalMolecular Reproduction and Development
    Issue number10
    StatePublished - Oct 2008


    • Corpus luteum
    • GnRH I and II and its receptors
    • Immunohistochemistry
    • Primates
    • RT-PCR and real-time PCR

    ASJC Scopus subject areas

    • Genetics
    • Developmental Biology
    • Cell Biology


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