Expression of VPAC2 receptor and PAC1 receptor splice variants in the trigeminal ganglion of the adult rat

Priya Chaudhary; Thomas K. Baumann

doi:10.1016/S0169-328X(02)00329-7

Expression of VPAC2 receptor and PAC1 receptor splice variants in the trigeminal ganglion of the adult rat

Priya Chaudhary
, Thomas K. Baumann

Neurology

Research output: Contribution to journal › Article › peer-review

38 Scopus citations

Abstract

PACAP and VIP are members of the VIP/secretin/glucagon family of peptides with neurotransmitter, neuroprotective, and neurotrophic functions. PACAP and VIP are known to be upregulated in primary sensory neurons following nerve injury, implying that these neuropeptides could be mediators of sensory transmission in neuropathic pain states. Nerve injury at the level of the trigeminal root is thought to be the prime cause of trigeminal neuralgia. Since cross-excitation (a chemically-mediated form of nonsynaptic transmission) within the TG is postulated to play a central role in trigeminal neuralgia, we studied the expression of PACAP and VIP receptors in the TG by RT PCR and immunocytochemistry. Of the three known receptors (PAC1, VPAC1 and VPAC2), RT PCR revealed the presence of mRNA for VPAC2 and several splice variants of the PAC1 receptor. Immunocytochemistry showed PAC1 and VPAC2 to be present in small-diameter TG neurons. Thus, PACAP and VIP are potential mediators of cross-excitation in the TG.

Original language	English (US)
Pages (from-to)	137-142
Number of pages	6
Journal	Molecular Brain Research
Volume	104
Issue number	2
DOIs	https://doi.org/10.1016/S0169-328X(02)00329-7
State	Published - 2002

Funding

This work was supported by the NRSA fellowship NS11043 to P.C. and the PHS grant NS37149 to T.K.B., both from the National Institute for Neurological Disease and Stroke. We would like to thank Dr. Victor May, University of Vermont, for generously sharing the antibody against PAC1 receptor.

Funders	Funder number
National Institute of Neurological Disorders and Stroke	F32NS011043

Keywords

PACAP
Trigeminal neuralgia
VIP

ASJC Scopus subject areas

Molecular Biology
Cellular and Molecular Neuroscience

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10.1016/S0169-328X(02)00329-7

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title = "Expression of VPAC2 receptor and PAC1 receptor splice variants in the trigeminal ganglion of the adult rat",

abstract = "PACAP and VIP are members of the VIP/secretin/glucagon family of peptides with neurotransmitter, neuroprotective, and neurotrophic functions. PACAP and VIP are known to be upregulated in primary sensory neurons following nerve injury, implying that these neuropeptides could be mediators of sensory transmission in neuropathic pain states. Nerve injury at the level of the trigeminal root is thought to be the prime cause of trigeminal neuralgia. Since cross-excitation (a chemically-mediated form of nonsynaptic transmission) within the TG is postulated to play a central role in trigeminal neuralgia, we studied the expression of PACAP and VIP receptors in the TG by RT PCR and immunocytochemistry. Of the three known receptors (PAC1, VPAC1 and VPAC2), RT PCR revealed the presence of mRNA for VPAC2 and several splice variants of the PAC1 receptor. Immunocytochemistry showed PAC1 and VPAC2 to be present in small-diameter TG neurons. Thus, PACAP and VIP are potential mediators of cross-excitation in the TG.",

keywords = "PACAP, Trigeminal neuralgia, VIP",

author = "Priya Chaudhary and Baumann, \{Thomas K.\}",

note = "Funding Information: This work was supported by the NRSA fellowship NS11043 to P.C. and the PHS grant NS37149 to T.K.B., both from the National Institute for Neurological Disease and Stroke. We would like to thank Dr. Victor May, University of Vermont, for generously sharing the antibody against PAC1 receptor.",

year = "2002",

doi = "10.1016/S0169-328X(02)00329-7",

language = "English (US)",

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AU - Baumann, Thomas K.

N1 - Funding Information: This work was supported by the NRSA fellowship NS11043 to P.C. and the PHS grant NS37149 to T.K.B., both from the National Institute for Neurological Disease and Stroke. We would like to thank Dr. Victor May, University of Vermont, for generously sharing the antibody against PAC1 receptor.

PY - 2002

Y1 - 2002

N2 - PACAP and VIP are members of the VIP/secretin/glucagon family of peptides with neurotransmitter, neuroprotective, and neurotrophic functions. PACAP and VIP are known to be upregulated in primary sensory neurons following nerve injury, implying that these neuropeptides could be mediators of sensory transmission in neuropathic pain states. Nerve injury at the level of the trigeminal root is thought to be the prime cause of trigeminal neuralgia. Since cross-excitation (a chemically-mediated form of nonsynaptic transmission) within the TG is postulated to play a central role in trigeminal neuralgia, we studied the expression of PACAP and VIP receptors in the TG by RT PCR and immunocytochemistry. Of the three known receptors (PAC1, VPAC1 and VPAC2), RT PCR revealed the presence of mRNA for VPAC2 and several splice variants of the PAC1 receptor. Immunocytochemistry showed PAC1 and VPAC2 to be present in small-diameter TG neurons. Thus, PACAP and VIP are potential mediators of cross-excitation in the TG.

AB - PACAP and VIP are members of the VIP/secretin/glucagon family of peptides with neurotransmitter, neuroprotective, and neurotrophic functions. PACAP and VIP are known to be upregulated in primary sensory neurons following nerve injury, implying that these neuropeptides could be mediators of sensory transmission in neuropathic pain states. Nerve injury at the level of the trigeminal root is thought to be the prime cause of trigeminal neuralgia. Since cross-excitation (a chemically-mediated form of nonsynaptic transmission) within the TG is postulated to play a central role in trigeminal neuralgia, we studied the expression of PACAP and VIP receptors in the TG by RT PCR and immunocytochemistry. Of the three known receptors (PAC1, VPAC1 and VPAC2), RT PCR revealed the presence of mRNA for VPAC2 and several splice variants of the PAC1 receptor. Immunocytochemistry showed PAC1 and VPAC2 to be present in small-diameter TG neurons. Thus, PACAP and VIP are potential mediators of cross-excitation in the TG.

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KW - Trigeminal neuralgia

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Expression of VPAC2 receptor and PAC1 receptor splice variants in the trigeminal ganglion of the adult rat

Abstract

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Keywords

ASJC Scopus subject areas

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