TY - JOUR
T1 - Fast and multiplexed superresolution imaging with DNA-PAINT-ERS
AU - Civitci, Fehmi
AU - Shangguan, Julia
AU - Zheng, Ting
AU - Tao, Kai
AU - Rames, Matthew
AU - Kenison, John
AU - Zhang, Ying
AU - Wu, Lei
AU - Phelps, Carey
AU - Esener, Sadik
AU - Nan, Xiaolin
N1 - Publisher Copyright:
© 2020, The Author(s).
PY - 2020/12/1
Y1 - 2020/12/1
N2 - DNA points accumulation for imaging in nanoscale topography (DNA-PAINT) facilitates multiplexing in superresolution microscopy but is practically limited by slow imaging speed. To address this issue, we propose the additions of ethylene carbonate (EC) to the imaging buffer, sequence repeats to the docking strand, and a spacer between the docking strand and the affinity agent. Collectively termed DNA-PAINT-ERS (E = EC, R = Repeating sequence, and S = Spacer), these strategies can be easily integrated into current DNA-PAINT workflows for both accelerated imaging speed and improved image quality through optimized DNA hybridization kinetics and efficiency. We demonstrate the general applicability of DNA-PAINT-ERS for fast, multiplexed superresolution imaging using previously validated oligonucleotide constructs with slight modifications.
AB - DNA points accumulation for imaging in nanoscale topography (DNA-PAINT) facilitates multiplexing in superresolution microscopy but is practically limited by slow imaging speed. To address this issue, we propose the additions of ethylene carbonate (EC) to the imaging buffer, sequence repeats to the docking strand, and a spacer between the docking strand and the affinity agent. Collectively termed DNA-PAINT-ERS (E = EC, R = Repeating sequence, and S = Spacer), these strategies can be easily integrated into current DNA-PAINT workflows for both accelerated imaging speed and improved image quality through optimized DNA hybridization kinetics and efficiency. We demonstrate the general applicability of DNA-PAINT-ERS for fast, multiplexed superresolution imaging using previously validated oligonucleotide constructs with slight modifications.
UR - http://www.scopus.com/inward/record.url?scp=85089973043&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85089973043&partnerID=8YFLogxK
U2 - 10.1038/s41467-020-18181-6
DO - 10.1038/s41467-020-18181-6
M3 - Article
C2 - 32859909
AN - SCOPUS:85089973043
SN - 2041-1723
VL - 11
JO - Nature communications
JF - Nature communications
IS - 1
M1 - 4339
ER -