TY - JOUR
T1 - Fetal lung development via quantitative biomarkers from diffusion MRI and histological validation in rhesus macaques
AU - Higano, Nara S.
AU - Cao, Xuefeng
AU - Guo, Jinbang
AU - Wang, Xiaojie
AU - Kroenke, Christopher D.
AU - Filuta, Alyssa L.
AU - Bridges, James P.
AU - Woods, Jason C.
N1 - Funding Information:
The authors thank the Oregon National Primate Research Center and the Imaging Research Center at Cincinnati Children’s Hospital. This publication was made possible, in part, using the Cincinnati Children’s Confocal Imaging Center; we specifically acknowledge Evan Meyer for assistance with tissue area-density measurements. NSH/XC/JG/XW/ALF prepared samples and performed experiments. CDK/JPB/JCW supervised work. NSH/XC wrote manuscript. All authors provided feedback and shaped analysis, interpretation, and manuscript. National Institutes of Health: R01 AA021981, R01 HL131634, and T32 HL007752. The Research Foundation at Cincinnati Children’s Hospital.
Publisher Copyright:
© 2021, The Author(s), under exclusive licence to Springer Nature America, Inc.
PY - 2022/7
Y1 - 2022/7
N2 - Objective: To demonstrate sensitivity of diffusion-weighted MRI (DW-MRI) to pulmonary cellular-space changes during normal in utero development using fetal rhesus macaques, compared to histological biomarkers. Study design: In vivo/ex vivo DW-MRI was acquired in 26 fetal rhesus lungs (early-canalicular through saccular stages). Apparent diffusion coefficients (ADC) from MRI and tissue area density (H&E), alveolar type-II cells (ABCA3), and epithelial cells (TTF1) from histology were compared between gestational stages. Results: In vivo/ex vivo ADC correlated with each other (Spearman ρ = 0.47, P = 0.038; Bland–Altman bias = 0.0835) and with area-density (in vivo ρ = -0.56, P = 0.011; ex vivo ρ = −0.83, P < 0.0001). In vivo/ex vivo ADC increased exponentially toward saturation with gestational stage (R2 = 0.49/0.49), while area-density decreased exponentially (R2 = 0.53). ABCA3 and TTF1 stains demonstrated expected fetal cellular development. Conclusions: Fetal DW-MRI provides a non-invasive biomarker for pulmonary structural maturation, with a strong correlation to histological markers during tissue development in rhesus macaques. This method has strong potential for assessing human fetal development, particularly in patients with pulmonary hypoplasia.
AB - Objective: To demonstrate sensitivity of diffusion-weighted MRI (DW-MRI) to pulmonary cellular-space changes during normal in utero development using fetal rhesus macaques, compared to histological biomarkers. Study design: In vivo/ex vivo DW-MRI was acquired in 26 fetal rhesus lungs (early-canalicular through saccular stages). Apparent diffusion coefficients (ADC) from MRI and tissue area density (H&E), alveolar type-II cells (ABCA3), and epithelial cells (TTF1) from histology were compared between gestational stages. Results: In vivo/ex vivo ADC correlated with each other (Spearman ρ = 0.47, P = 0.038; Bland–Altman bias = 0.0835) and with area-density (in vivo ρ = -0.56, P = 0.011; ex vivo ρ = −0.83, P < 0.0001). In vivo/ex vivo ADC increased exponentially toward saturation with gestational stage (R2 = 0.49/0.49), while area-density decreased exponentially (R2 = 0.53). ABCA3 and TTF1 stains demonstrated expected fetal cellular development. Conclusions: Fetal DW-MRI provides a non-invasive biomarker for pulmonary structural maturation, with a strong correlation to histological markers during tissue development in rhesus macaques. This method has strong potential for assessing human fetal development, particularly in patients with pulmonary hypoplasia.
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U2 - 10.1038/s41372-021-01236-x
DO - 10.1038/s41372-021-01236-x
M3 - Article
C2 - 34686834
AN - SCOPUS:85117608938
SN - 0743-8346
VL - 42
SP - 866
EP - 872
JO - Journal of Perinatology
JF - Journal of Perinatology
IS - 7
ER -