Flow infusion electrospray ionization high-resolution mass spectrometry of the chloride adducts of sphingolipids, glycerophospholipids, glycerols, hydroxy fatty acids, fatty acid esters of hydroxy fatty acids (FAHFA), and modified nucleosides

Paul L. Wood; Randall L. Woltjer

doi:10.1007/978-1-0716-0864-7_6

Flow infusion electrospray ionization high-resolution mass spectrometry of the chloride adducts of sphingolipids, glycerophospholipids, glycerols, hydroxy fatty acids, fatty acid esters of hydroxy fatty acids (FAHFA), and modified nucleosides

Paul L. Wood, Randall L. Woltjer

Pathology

Research output: Chapter in Book/Report/Conference proceeding › Chapter

1 Scopus citations

Abstract

Ceramides are generally monitored via either their anions or cations formed with electrospray ionization (ESI). While these methods appear adequate for chromatographic assays, they offer limited sensitivity with flow infusion ESI (“shotgun lipidomics”). We therefore evaluated the chloride adducts [M + Cl]⁻ of a number of lipid classes and found 10- to 50-fold increases in assay sensitivity for ceramides, hexosylceramides, lactosylceramides, phytoceramides, dihydroceramides, and ceramide phosphoethanolamines. Chloride adducts of glycerophosphocholines, sphingomyelins, hydroxyl fatty acids, and modified nucleotides also were characterized. Chloride adducts of glycerophosphocholines were particularly useful for the MS/MS analysis of the constituent fatty acids at sn-1 and sn-2.

Original language	English (US)
Title of host publication	Neuromethods
Publisher	Humana Press Inc.
Pages	69-76
Number of pages	8
DOIs	https://doi.org/10.1007/978-1-0716-0864-7_6
State	Published - 2021

Publication series

Name	Neuromethods
Volume	159
ISSN (Print)	0893-2336
ISSN (Electronic)	1940-6045

Keywords

Ceramides
Chloride adducts
Negative ion electrospray mass spectrometry

ASJC Scopus subject areas

Neuroscience(all)
Biochemistry, Genetics and Molecular Biology(all)
Pharmacology, Toxicology and Pharmaceutics(all)
Psychiatry and Mental health

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Wood, P. L., & Woltjer, R. L. (2021). Flow infusion electrospray ionization high-resolution mass spectrometry of the chloride adducts of sphingolipids, glycerophospholipids, glycerols, hydroxy fatty acids, fatty acid esters of hydroxy fatty acids (FAHFA), and modified nucleosides. In Neuromethods (pp. 69-76). (Neuromethods; Vol. 159). Humana Press Inc.. https://doi.org/10.1007/978-1-0716-0864-7_6

Flow infusion electrospray ionization high-resolution mass spectrometry of the chloride adducts of sphingolipids, glycerophospholipids, glycerols, hydroxy fatty acids, fatty acid esters of hydroxy fatty acids (FAHFA), and modified nucleosides. / Wood, Paul L.; Woltjer, Randall L.
Neuromethods. Humana Press Inc., 2021. p. 69-76 (Neuromethods; Vol. 159).

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Wood, PL & Woltjer, RL 2021, Flow infusion electrospray ionization high-resolution mass spectrometry of the chloride adducts of sphingolipids, glycerophospholipids, glycerols, hydroxy fatty acids, fatty acid esters of hydroxy fatty acids (FAHFA), and modified nucleosides. in Neuromethods. Neuromethods, vol. 159, Humana Press Inc., pp. 69-76. https://doi.org/10.1007/978-1-0716-0864-7_6

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Flow infusion electrospray ionization high-resolution mass spectrometry of the chloride adducts of sphingolipids, glycerophospholipids, glycerols, hydroxy fatty acids, fatty acid esters of hydroxy fatty acids (FAHFA), and modified nucleosides

Abstract

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ASJC Scopus subject areas

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