Flow karyotyping: current status and potential development

M. A. Van Dilla; A. V. Carrano; J. W. Gray

Flow karyotyping: current status and potential development

M. A. Van Dilla, A. V. Carrano, J. W. Gray

Research output: Contribution to journal › Article › peer-review

Abstract

Suspensions of metaphase chromosomes from chinese hamster, Indian muntjac and human tissue culture cells were stained for DNA with the fluorochrome, ethidium bromide, and analyzed by flow cytometry to produce a DNA distribution or flow karyotype unique for each species. The flow karyotype is a representation of chromosomal DNA content and relative chromosome frequency. The time for the measurement and data reduction is about ten minutes per 10⁶ chromosomes. Aberrant chromosomes have been detected by flow karyotype changes. Improvements of flow karyotyping and the feasibility of this technique for monitoring human populations for cytogenetic alterations are discussed.

Original language	English (US)
Pages (from-to)	145-164
Number of pages	20
Journal	LAWRENCE LIVERMORE LAB., U.CALIF.
State	Published - 1976
Externally published	Yes

ASJC Scopus subject areas

General Medicine

Cite this

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title = "Flow karyotyping: current status and potential development",

abstract = "Suspensions of metaphase chromosomes from chinese hamster, Indian muntjac and human tissue culture cells were stained for DNA with the fluorochrome, ethidium bromide, and analyzed by flow cytometry to produce a DNA distribution or flow karyotype unique for each species. The flow karyotype is a representation of chromosomal DNA content and relative chromosome frequency. The time for the measurement and data reduction is about ten minutes per 106 chromosomes. Aberrant chromosomes have been detected by flow karyotype changes. Improvements of flow karyotyping and the feasibility of this technique for monitoring human populations for cytogenetic alterations are discussed.",

author = "{Van Dilla}, {M. A.} and Carrano, {A. V.} and Gray, {J. W.}",

year = "1976",

language = "English (US)",

pages = "145--164",

journal = "LAWRENCE LIVERMORE LAB., U.CALIF.",

}

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T1 - Flow karyotyping

T2 - current status and potential development

AU - Van Dilla, M. A.

AU - Carrano, A. V.

AU - Gray, J. W.

PY - 1976

Y1 - 1976

N2 - Suspensions of metaphase chromosomes from chinese hamster, Indian muntjac and human tissue culture cells were stained for DNA with the fluorochrome, ethidium bromide, and analyzed by flow cytometry to produce a DNA distribution or flow karyotype unique for each species. The flow karyotype is a representation of chromosomal DNA content and relative chromosome frequency. The time for the measurement and data reduction is about ten minutes per 106 chromosomes. Aberrant chromosomes have been detected by flow karyotype changes. Improvements of flow karyotyping and the feasibility of this technique for monitoring human populations for cytogenetic alterations are discussed.

AB - Suspensions of metaphase chromosomes from chinese hamster, Indian muntjac and human tissue culture cells were stained for DNA with the fluorochrome, ethidium bromide, and analyzed by flow cytometry to produce a DNA distribution or flow karyotype unique for each species. The flow karyotype is a representation of chromosomal DNA content and relative chromosome frequency. The time for the measurement and data reduction is about ten minutes per 106 chromosomes. Aberrant chromosomes have been detected by flow karyotype changes. Improvements of flow karyotyping and the feasibility of this technique for monitoring human populations for cytogenetic alterations are discussed.

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JO - LAWRENCE LIVERMORE LAB., U.CALIF.

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ER -

Flow karyotyping: current status and potential development

Abstract

ASJC Scopus subject areas

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