TY - JOUR
T1 - Fluorescent nucleic add hybridization methods
AU - Pinkel, D.
AU - Gray, J.
AU - Segraves, R.
AU - Waldman, F.
AU - Trask, B.
AU - Yu, L. C.
AU - Eastmond, D.
AU - Dean, P.
N1 - Funding Information:
Work performed under the auspices of the U.S. Department of Energy by the Lawrence Livermore National Laboratory under contract number W-7405-ENG-48 with support from USPHS Grants HD 17655 and CA 45919.
PY - 1989/6/13
Y1 - 1989/6/13
N2 - In the last several years a number of approaches have been developed that permit detection of nucleic acid hybridization using fluorescence techniques. These have several advantages over the previously available radioactive procedures. Among these are much higher spatial resolution, the speed with which the results are available, the reduced hazard, and the possibility of distinguishing the binding of multiple probes using different fluorochromes. Exploitation of this potential requires application of optical techniques of increasing sophistication such as development of new fluorophores, improved methods of visualizing multiple dyes in the same sample, application of image processing to extract quantitative information, and optimized optical sectioning for three dimensional reconstructions.
AB - In the last several years a number of approaches have been developed that permit detection of nucleic acid hybridization using fluorescence techniques. These have several advantages over the previously available radioactive procedures. Among these are much higher spatial resolution, the speed with which the results are available, the reduced hazard, and the possibility of distinguishing the binding of multiple probes using different fluorochromes. Exploitation of this potential requires application of optical techniques of increasing sophistication such as development of new fluorophores, improved methods of visualizing multiple dyes in the same sample, application of image processing to extract quantitative information, and optimized optical sectioning for three dimensional reconstructions.
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U2 - 10.1117/12.951898
DO - 10.1117/12.951898
M3 - Article
AN - SCOPUS:84455177494
SN - 0277-786X
VL - 1063
SP - 123
EP - 132
JO - Proceedings of SPIE - The International Society for Optical Engineering
JF - Proceedings of SPIE - The International Society for Optical Engineering
ER -