Fluorescent nucleic add hybridization methods

D. Pinkel, J. Gray, R. Segraves, F. Waldman, B. Trask, L. C. Yu, D. Eastmond, P. Dean

Research output: Contribution to journalArticlepeer-review

1 Scopus citations


In the last several years a number of approaches have been developed that permit detection of nucleic acid hybridization using fluorescence techniques. These have several advantages over the previously available radioactive procedures. Among these are much higher spatial resolution, the speed with which the results are available, the reduced hazard, and the possibility of distinguishing the binding of multiple probes using different fluorochromes. Exploitation of this potential requires application of optical techniques of increasing sophistication such as development of new fluorophores, improved methods of visualizing multiple dyes in the same sample, application of image processing to extract quantitative information, and optimized optical sectioning for three dimensional reconstructions.

Original languageEnglish (US)
Pages (from-to)123-132
Number of pages10
JournalProceedings of SPIE - The International Society for Optical Engineering
StatePublished - Jun 13 1989
Externally publishedYes

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering


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