Fucoidan and topography modification improved in situ endothelialization on acellular synthetic vascular grafts

Yuan Yao, Aung Moe Zaw, Deirdre E.J. Anderson, Ye Jin Jeong, Joshua Kunihiro, Monica T. Hinds, Evelyn K.F. Yim

Research output: Contribution to journalArticlepeer-review

9 Scopus citations


Thrombogenesis remains the primary failure of synthetic vascular grafts. Endothelial coverage is crucial to provide an antithrombogenic surface. However, most synthetic materials do not support cell adhesion, and transanastomotic endothelial migration is limited. Here, a surface modification strategy using fucoidan and topography was developed to enable fast in situ endothelialization of polyvinyl alcohol, which is not endothelial cell-adhesive. Among three different immobilization approaches compared, conjugation of aminated-fucoidan promoted endothelial monolayer formation while minimizing thrombogenicity in both in vitro platelet rich plasma testing and ex vivo non-human primate shunt assay. Screening of six topographical patterns showed that 2 μm gratings increased endothelial cell migration without inducing inflammation responses of endothelial cells. Mechanistic studies demonstrated that fucoidan could attract fibronectin, enabling integrin binding and focal adhesion formation and activating focal adhesion kinase (FAK) signaling, and 2 μm gratings further enhanced FAK-mediated cell migration. In a clinically relevant rabbit carotid artery end-to-side anastomosis model, 60% in situ endothelialization was observed throughout the entire lumen of 1.7 mm inner diameter modified grafts, compared to 0% of unmodified graft, and the four-week graft patency also increased. This work presents a promising strategy to stimulate in situ endothelialization on synthetic materials for improving long-term performance.

Original languageEnglish (US)
Pages (from-to)535-550
Number of pages16
JournalBioactive Materials
StatePublished - Apr 2023


  • Blood-contacting devices
  • Endothelialization
  • Fucoidan
  • Small diameter vascular graft
  • Topography

ASJC Scopus subject areas

  • Biotechnology
  • Biomaterials
  • Biomedical Engineering


Dive into the research topics of 'Fucoidan and topography modification improved in situ endothelialization on acellular synthetic vascular grafts'. Together they form a unique fingerprint.

Cite this