Full genome sequence analysis of a novel adenovirus of rhesus macaque origin indicates a new simian adenovirus type and species

Daniel Malouli; Grant L. Howell; Alfred W. Legasse; Christoph Kahl; Michael K. Axthelm; Scott G. Hansen; Klaus Früh

doi:10.1016/j.virep.2014.10.001

Full genome sequence analysis of a novel adenovirus of rhesus macaque origin indicates a new simian adenovirus type and species

Daniel Malouli
, Grant L. Howell
, Alfred W. Legasse
, Christoph Kahl
, Michael K. Axthelm
, Scott G. Hansen
, Klaus Früh

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Multiple novel simian adenoviruses have been isolated over the past years and their potential to cross the species barrier and infect the human population is an ever present threat. Here we describe the isolation and full genome sequencing of a novel simian adenovirus (SAdV) isolated from the urine of two independent, never co-housed, late stage simian immunodeficiency virus (SIV)-infected rhesus macaques. The viral genome sequences revealed a novel type with a unique genome length, GC content, E3 region and DNA polymerase amino acid sequence that is sufficiently distinct from all currently known human- or simian adenovirus species to warrant classifying these isolates as a novel species of simian adenovirus. This new species, termed Simian mastadenovirus D (SAdV-D), displays the standard genome organization for the genus Mastadenovirus containing only one copy of the fiber gene which sets it apart from the old world monkey adenovirus species HAdV-G, SAdV-B and SAdV-C.

Original language	English (US)
Pages (from-to)	18-29
Number of pages	12
Journal	Virology Reports
Volume	3
DOIs	https://doi.org/10.1016/j.virep.2014.10.001
State	Published - Sep 1 2014

Funding

This project was supported by the National Center for Research Resources and the Office of Research Infrastructure Programs (ORIP) of the National Institutes of Health through grant P51OD011092 . We are grateful to Dr. Robert Searles and the OHSU Massively Parallel Sequencing Shared Resource (MPSSR) for generating the NGS libraries, to Yibing Jia and the Molecular and Cellular Biology Core (MCB Core) at the Oregon National Primate Research Center (ONPRC) for analyzing the samples on their MiSeq next generation sequencer and to Andrew Townsend for his help in the graphical design and preparation of the here presented figures.

Funders	Funder number
Author National Institutes of Health National Institutes of Health National Institutes of Health National Institutes of Health The Bev Hartig Huntington's Disease Foundation National Institutes of Health	P51OD011092
National Center for Research Resources
National Institutes of Health Office of Research Infrastructure Programs

Keywords

Adenovirus
Adenovirus species
Non-human primate
Rhesus macaque
Simian adenovirus
Virus genome analysis
Virus phylogenomics

ASJC Scopus subject areas

Biochemistry
Virology

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10.1016/j.virep.2014.10.001

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title = "Full genome sequence analysis of a novel adenovirus of rhesus macaque origin indicates a new simian adenovirus type and species",

abstract = "Multiple novel simian adenoviruses have been isolated over the past years and their potential to cross the species barrier and infect the human population is an ever present threat. Here we describe the isolation and full genome sequencing of a novel simian adenovirus (SAdV) isolated from the urine of two independent, never co-housed, late stage simian immunodeficiency virus (SIV)-infected rhesus macaques. The viral genome sequences revealed a novel type with a unique genome length, GC content, E3 region and DNA polymerase amino acid sequence that is sufficiently distinct from all currently known human- or simian adenovirus species to warrant classifying these isolates as a novel species of simian adenovirus. This new species, termed Simian mastadenovirus D (SAdV-D), displays the standard genome organization for the genus Mastadenovirus containing only one copy of the fiber gene which sets it apart from the old world monkey adenovirus species HAdV-G, SAdV-B and SAdV-C.",

keywords = "Adenovirus, Adenovirus species, Non-human primate, Rhesus macaque, Simian adenovirus, Virus genome analysis, Virus phylogenomics",

author = "Daniel Malouli and Howell, \{Grant L.\} and Legasse, \{Alfred W.\} and Christoph Kahl and Axthelm, \{Michael K.\} and Hansen, \{Scott G.\} and Klaus Fr{\"u}h",

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doi = "10.1016/j.virep.2014.10.001",

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AU - Kahl, Christoph

AU - Axthelm, Michael K.

AU - Hansen, Scott G.

AU - Früh, Klaus

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N2 - Multiple novel simian adenoviruses have been isolated over the past years and their potential to cross the species barrier and infect the human population is an ever present threat. Here we describe the isolation and full genome sequencing of a novel simian adenovirus (SAdV) isolated from the urine of two independent, never co-housed, late stage simian immunodeficiency virus (SIV)-infected rhesus macaques. The viral genome sequences revealed a novel type with a unique genome length, GC content, E3 region and DNA polymerase amino acid sequence that is sufficiently distinct from all currently known human- or simian adenovirus species to warrant classifying these isolates as a novel species of simian adenovirus. This new species, termed Simian mastadenovirus D (SAdV-D), displays the standard genome organization for the genus Mastadenovirus containing only one copy of the fiber gene which sets it apart from the old world monkey adenovirus species HAdV-G, SAdV-B and SAdV-C.

AB - Multiple novel simian adenoviruses have been isolated over the past years and their potential to cross the species barrier and infect the human population is an ever present threat. Here we describe the isolation and full genome sequencing of a novel simian adenovirus (SAdV) isolated from the urine of two independent, never co-housed, late stage simian immunodeficiency virus (SIV)-infected rhesus macaques. The viral genome sequences revealed a novel type with a unique genome length, GC content, E3 region and DNA polymerase amino acid sequence that is sufficiently distinct from all currently known human- or simian adenovirus species to warrant classifying these isolates as a novel species of simian adenovirus. This new species, termed Simian mastadenovirus D (SAdV-D), displays the standard genome organization for the genus Mastadenovirus containing only one copy of the fiber gene which sets it apart from the old world monkey adenovirus species HAdV-G, SAdV-B and SAdV-C.

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KW - Non-human primate

KW - Rhesus macaque

KW - Simian adenovirus

KW - Virus genome analysis

KW - Virus phylogenomics

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Full genome sequence analysis of a novel adenovirus of rhesus macaque origin indicates a new simian adenovirus type and species

Abstract

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Keywords

ASJC Scopus subject areas

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