Functional and phenotypic analysis of human memory CD8+ T cells expressing CXCR3

Naoki Kobayashi, Takaaki Kondo, Hiroshi Takata, Shumpei Yokota, Masafumi Takiguchi

Research output: Contribution to journalArticlepeer-review

25 Scopus citations


Several chemokine receptors play an important role in the migration of naïve, memory, and effector T cells. Flow cytometric analyses showed that human CD8+ T cells with naïve (CD27+CD28 +CD45RA+) or memory (CD27+CD28 +/-CD45RA+) phenotypes included a population expressing a high level of CXC chemokine receptor 3 (CXCR3high) and one expressing a low level of it (CXCR3low), but those with the effector phenotype (CD27-CD28-CD45RA+/-) included a population that did not express CXCR3 (CXCR3-) and a CXCR3low population. This relation between the expression level of CXCR3 and memory/effector phenotypes also applied to Epstein-Barr virus- or human cytomegalovirus-specific CD8+ T cells. CXCR3high cells were found predominantly in CC chemokine receptor 7 (CCR7) +CCR5- and CCR7-CCR5- subsets of CD8+ T cells with the CD27+CD28+CD45RA - memory phenotype, suggesting that they are memory cells with intermediate differentiation. Indeed, CXCR3highCD27 +CD28+CD45RA--CD8+ T cells had the ability to produce interleukin-2 and interferon-γ. These results together indicate that the expression of CXCR3 is upregulated on intermediately differentiated memory CD8+ T cells. CXCR3highCD8 + T cells had a greater ability to migrate in response to CXCR3 ligands than CXCR3low ones. As CXCR3high memory CD8 + T cells do not express CCR5, high expression of CXCR3 on these memory CD8+ T cells might play an important role in the migration of these cells to inflammatory sites and in their differentiation.

Original languageEnglish (US)
Pages (from-to)320-329
Number of pages10
JournalJournal of Leukocyte Biology
Issue number2
StatePublished - Aug 2006
Externally publishedYes


  • Chemokine receptor
  • Cytokine
  • Differentiation

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Cell Biology


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