Dissociated cell cultures of the rodent hippocampus have become a standard model for studying many facets of neural development, including the development of polarity, axonal and dendritic growth, and synapse formation. The cultures are quite homogeneous-~90% of the cells are pyramidal neurons- and it is relatively easy to express green fluorescent protein (GFP)-tagged proteins by transfection. This article describes the cultures and the key features of the system used to image them. It also includes suggestions on labeling cells with GFP-tagged proteins. It concludes with a discussion of the advantages and disadvantages of this culture system.
|Original language||English (US)|
|Number of pages||7|
|Journal||Cold Spring Harbor Protocols|
|State||Published - Mar 2012|
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)