Generation of coated intermediates of clathrin-mediated endocytosis on protein-free liposomes

Kohji Takei, Volker Haucke, Vladimir Slepnev, Khashayar Farsad, Marco Salazar, Hong Chen, Pietro De Camilli

Research output: Contribution to journalArticlepeer-review

298 Scopus citations


Clathrin-coated buds and dynamin-coated tubules morphologically similar to corresponding structures observed in synaptic membranes can be generated on protein-free liposomes by incubation with cytosol, or with clathrin coat proteins and purified dynamin, respectively. Dynamin- and clathrin-coated intermediates may form independently of each other, despite the coupling between the two processes typically observed in synaptic membranes. Formation of both structures on liposomes can occur in the absence of nucleotides. These findings indicate that interfaces between lipids and cytosolic proteins are fully sufficient to deform lipids bilayers into buds and tubules. They suggest that a main function of membrane proteins is to act as positive and negative regulators of coat assembly, therefore controlling these processes in time and space.

Original languageEnglish (US)
Pages (from-to)131-141
Number of pages11
Issue number1
StatePublished - Jul 10 1998
Externally publishedYes

ASJC Scopus subject areas

  • General Biochemistry, Genetics and Molecular Biology


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