Genetic diversity of plaque mutans streptococci with rep-PCR

K. Cheon; S. A. Moser; J. Whiddon; R. C. Osgood; S. Momeni; J. D. Ruby; G. R. Cutter; D. B. Allison; N. K. Childers

doi:10.1177/0022034510386375

Genetic diversity of plaque mutans streptococci with rep-PCR

K. Cheon, S. A. Moser, J. Whiddon, R. C. Osgood, S. Momeni, J. D. Ruby, G. R. Cutter, D. B. Allison, N. K. Childers

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

Mutans streptococci (MS) are key organisms associated with the etiology of dental caries. Using probabilities that were tested by oversampling, we designed this study to determine the minimal number of MS isolates from an individual required to evaluate diversity of genotypes. MS isolates were genotyped by repetitive extragenic palindromic-polymerase chain-reaction (rep-PCR). Analysis of 20 isolates from individuals resulted in a mean of 1.6 and 2.4 genotypes in children (N = 12) and adults (N = 10), respectively. In a follow-up study, reducing the number of isolates to 7-10 resulted in a theoretical probability of up to 78% for detecting up to 4 genotypes. A mean of 1.5 genotypes was found in 35 children and 10 adults. These findings provide evidence for the design of studies of MS genotyping that can serve as a model for the analysis of genotypes within individuals.

Original language	English (US)
Pages (from-to)	331-335
Number of pages	5
Journal	Journal of dental research
Volume	90
Issue number	3
DOIs	https://doi.org/10.1177/0022034510386375
State	Published - Mar 2011
Externally published	Yes

Keywords

biostatistics
caries
genotypes
microbial genetics
plaque/plaquebiofilms
Streptococcus mutans

ASJC Scopus subject areas

General Dentistry

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10.1177/0022034510386375

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title = "Genetic diversity of plaque mutans streptococci with rep-PCR",

abstract = "Mutans streptococci (MS) are key organisms associated with the etiology of dental caries. Using probabilities that were tested by oversampling, we designed this study to determine the minimal number of MS isolates from an individual required to evaluate diversity of genotypes. MS isolates were genotyped by repetitive extragenic palindromic-polymerase chain-reaction (rep-PCR). Analysis of 20 isolates from individuals resulted in a mean of 1.6 and 2.4 genotypes in children (N = 12) and adults (N = 10), respectively. In a follow-up study, reducing the number of isolates to 7-10 resulted in a theoretical probability of up to 78% for detecting up to 4 genotypes. A mean of 1.5 genotypes was found in 35 children and 10 adults. These findings provide evidence for the design of studies of MS genotyping that can serve as a model for the analysis of genotypes within individuals.",

keywords = "biostatistics, caries, genotypes, microbial genetics, plaque/plaquebiofilms, Streptococcus mutans",

author = "K. Cheon and Moser, {S. A.} and J. Whiddon and Osgood, {R. C.} and S. Momeni and Ruby, {J. D.} and Cutter, {G. R.} and Allison, {D. B.} and Childers, {N. K.}",

year = "2011",

month = mar,

doi = "10.1177/0022034510386375",

language = "English (US)",

volume = "90",

pages = "331--335",

journal = "Journal of dental research",

issn = "0022-0345",

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AU - Cheon, K.

AU - Moser, S. A.

AU - Whiddon, J.

AU - Osgood, R. C.

AU - Momeni, S.

AU - Ruby, J. D.

AU - Cutter, G. R.

AU - Allison, D. B.

AU - Childers, N. K.

PY - 2011/3

Y1 - 2011/3

N2 - Mutans streptococci (MS) are key organisms associated with the etiology of dental caries. Using probabilities that were tested by oversampling, we designed this study to determine the minimal number of MS isolates from an individual required to evaluate diversity of genotypes. MS isolates were genotyped by repetitive extragenic palindromic-polymerase chain-reaction (rep-PCR). Analysis of 20 isolates from individuals resulted in a mean of 1.6 and 2.4 genotypes in children (N = 12) and adults (N = 10), respectively. In a follow-up study, reducing the number of isolates to 7-10 resulted in a theoretical probability of up to 78% for detecting up to 4 genotypes. A mean of 1.5 genotypes was found in 35 children and 10 adults. These findings provide evidence for the design of studies of MS genotyping that can serve as a model for the analysis of genotypes within individuals.

AB - Mutans streptococci (MS) are key organisms associated with the etiology of dental caries. Using probabilities that were tested by oversampling, we designed this study to determine the minimal number of MS isolates from an individual required to evaluate diversity of genotypes. MS isolates were genotyped by repetitive extragenic palindromic-polymerase chain-reaction (rep-PCR). Analysis of 20 isolates from individuals resulted in a mean of 1.6 and 2.4 genotypes in children (N = 12) and adults (N = 10), respectively. In a follow-up study, reducing the number of isolates to 7-10 resulted in a theoretical probability of up to 78% for detecting up to 4 genotypes. A mean of 1.5 genotypes was found in 35 children and 10 adults. These findings provide evidence for the design of studies of MS genotyping that can serve as a model for the analysis of genotypes within individuals.

KW - biostatistics

KW - caries

KW - genotypes

KW - microbial genetics

KW - plaque/plaquebiofilms

KW - Streptococcus mutans

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ER -

Genetic diversity of plaque mutans streptococci with rep-PCR

Abstract

Keywords

ASJC Scopus subject areas

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